Amplification of Rad9 in head and neck cancer

Rad9 在头颈癌中的扩增

• 批准号: 20592026
• 负责人: ISHIKAWA Kazuhiro
• 金额: $ 2.91万
• 依托单位: Jichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592026/
• 关键词: 癌幹細胞; 癌治療

In the recent years, evidence is increasing indicating the existence of a subpopulation of resistant tumor cells in head and neck squamous cell carcinoma that cannot be eradicated by chemotherapy or radiotherapy. Human Rad9 is involved in cell cycle checkpoints, repair of damaged DNA, and apoptosis. Previous studies suggest that Rad9 is frequently amplified in epithelial tumor cells of head and neck cancer, but little is known about its role in the development of head and neck cancer. Here we studied the direct association of Rad9 and p53 results in regulation of the expression of P21 gene. Immunohistochemistrical analysis in head and neck cancer detected the accumulation of Rad9 in the nuclei of tumor cells, which colocalized with p53. In addition, molecular biological studies revealed the direct association of Rad9 and Rev7, an error-prone DNA polymerase. Collectively, these findings suggest that hRad9 may play a role in genetic instability and carcinogenesis. Further experiments might reveal its role as a diagnostic and prognostic factor, and possibly as a therapeutic target.

近年来，越来越多的证据表明，头颈部鳞状细胞癌中存在一种不能通过化疗或放疗根除的耐药肿瘤细胞亚群。人类Rad9参与细胞周期检查点、受损DNA的修复和细胞凋亡。既往研究提示Rad9在头颈癌上皮肿瘤细胞中频繁扩增，但对其在头颈癌发生发展中的作用知之甚少。我们研究了Rad9和p53在P21基因表达调控中的直接关联。免疫组化分析在头颈部肿瘤中检测到肿瘤细胞核中Rad9的积累，Rad9与p53共定位。此外，分子生物学研究揭示了Rad9和Rev7（一种容易出错的DNA聚合酶）的直接关联。总之，这些发现表明hRad9可能在遗传不稳定和致癌中起作用。进一步的实验可能会揭示其作为诊断和预后因素的作用，并可能作为治疗靶点。

项目成果

頭頸部癌におけるRad9発現

Rad9在头颈癌中的表达

• 发表时间: 2008
• 期刊: 頭頸部癌 34巻
• 作者: 石川和宏;西野宏;市村恵一;石井秀始
• 通讯作者: 石井秀始

頭頸部癌におけるRad9の発現の検討

头颈癌中 Rad9 表达的检测

• 发表时间: 2008
• 作者: 石川和宏;西野宏;市村恵一;石井秀始
• 通讯作者: 石井秀始

頭頸部癌におけるRad9の発現の免疫組織学的検討

头颈癌中Rad9表达的免疫组织学检查

• 发表时间: 2008
• 作者: 石川和宏;西野宏;市村恵一;石井秀始
• 通讯作者: 石井秀始