Immunolocalization of hormone receptors and their regulatory system in the hormonally responsive ducts of the mouse salivary glands

小鼠唾液腺激素反应管中激素受体及其调节系统的免疫定位

• 批准号: 20592158
• 负责人: KURABUCHI Shingo
• 金额: $ 3.24万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592158/
• 关键词: 唾液腺; 顆粒性導管; アンドロゲン; 甲状腺ホルモン; ホルモン受容体; 免疫組織化学; 表現型; マウス; Androgen; 電子顕微鏡

Granular convoluted tubule (GCT) -like cells in the submandibular gland (SMG), were induced in the striated ducts (SDs) of the parotid gland (PAG) of mouse after the repeatedly injections of androgen and thyroid hormone. This finding and our serial studies suggest that the hormone-dependent GCT is a common characterization in murine three major salivary glands. However, the process of cytodifferentiation from immature granular cells to GCT cells will be varying in each gland. This process is completed in a large population of SD cells in the SMG, with a small population restricted to the sublingual gland (SLG), but all of the SD cells maintain their original form in the PAG. The gland-specific GCT-phenotype might be caused by contents of hormone receptors. However, we have tried unsuccessfully to detect immunolocalization and western blotting of androgen receptor (AR) in the mouse salivary glands, using several commercially available antibodies for ARs. It is conceivable that molecular structures of ARs may be different between the salivary glands and the reproductive organs.

反复注射雄激素和甲状腺激素后，在小鼠腮腺纹状管（SD）内诱导出颗粒曲管（GCT）样细胞。这一发现和我们的系列研究表明，依赖于唾液酸的GCT是一个共同的特点，在小鼠的三个主要唾液腺。然而，从未成熟颗粒细胞到GCT细胞的细胞分化过程在每个腺体中是不同的。这一过程在SMG中的大量SD细胞中完成，其中一小部分局限于舌下腺（SLG），但所有SD细胞在PAG中保持其原始形式。腺体特异性GCT表型可能是由激素受体的含量引起的。然而，我们已经尝试不成功地检测免疫定位和免疫印迹的雄激素受体（AR）在小鼠唾液腺中，使用几个市售的抗体的AR。可以想象，唾液腺和生殖器官中AR的分子结构可能不同。

项目成果

Characterization of hormone-responsible GCT-like cells in the parotid gland of mice

小鼠腮腺中激素依赖性 GCT 样细胞的表征

• 发表时间: 2009
• 作者: Kurabuchi S;Hosoi K
• 通讯作者: Hosoi K

Expression of a mammalian aquaporin 3 homolog in the anterior pituitary gonadotrophs of the tree frog, Hyla japonica

哺乳动物水通道蛋白 3 同源物在树蛙垂体前叶促性腺激素中的表达

• DOI: 10.1007/s00441-010-1122-1
• 发表时间: 2011
• 期刊: Cell Tissue Res.
• 作者: Sato M;Nakakura T;Ogushi Y;Akabane G;Kurabuchi S;Suzuki M;Tanaka S
• 通讯作者: Tanaka S

Expression of a mammalian aquaporin 3 ho-molog in the anterior pituitary gonadotrophs of the tree frog

哺乳动物水通道蛋白 3 同源物在树蛙垂体前叶促性腺激素中的表达

• 发表时间: 2011
• 期刊: Hyla japonica.(Cell tissue Research) 343
• 作者: Sato S;Nakamura T;Ogushi Y;Akabane G;Kurabuchi S;Suzuki M;Tanaka S
• 通讯作者: Tanaka S

Immunocytochemical study of granular duct cells with a hormonally enhanced granular cell phenotype in the mouse parotid gland

小鼠腮腺中激素增强颗粒细胞表型的颗粒导管细胞的免疫细胞化学研究

• 发表时间: 2009
• 期刊: Odontology 97
• 作者: Kurabuchi S;Hosoi K
• 通讯作者: Hosoi K

唾液腺の細胞組織学的研究と臨床応用

唾液腺的细胞组织学研究及临床应用

• 发表时间: 2010
• 期刊: 歯学特集号「研究成果と臨床応用」
• 作者: 相山誉夫;倉淵眞悟;池田理恵;菊池憲一郎;高田清美;佐藤住美江
• 通讯作者: 佐藤住美江