Elucidation of mechanisms underlying regulation of proliferation and differentiation of mesenchymal stem cells derived from periodontal ligament

阐明牙周膜来源的间充质干细胞增殖和分化的调控机制

• 批准号: 20592169
• 负责人: ISHISAKI Akira
• 金额: $ 3万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592169/
• 关键词: 幹細胞; 歯根膜; 再生医療; 再生医学; 間葉系幹細胞; 転写因子; 歯学; 細胞・組織; 血管内皮細胞; 骨芽細胞; 細胞分化

To elucidate molecular mechanisms underlying the regulation of proliferation and differentiation of mesenchymal stem cells (MSCs) derived from periodontal ligament (PDL), we established two types of single cell-derived cultures (SCDCs) of rat ligament cells, one of which named as SCDC2 retained the ability of self-renewal and multipotency and another of which named as SCDC1 did not. Then, we comprehensively compared the genes expression status and phosphorylation status of intracellular molecules between SCDC1 and SCDC2. We found that among 54,000 target genes, around 600 genes were identified as differentially expressed genes between the two types of cells. In particular, we found that some transcription factors, which were previously reported as proliferation and differentiation regulators of several kinds of stem cells, were highly expressed in the SCDC2 cells. Moreover, we found that the FGF-induced- and TGF--induced-intracellular signaling played important roles on the regulation of proliferation and differentiation of the MSCs. Thus, we successfully elucidated the molecular mechanisms underlying the regulation of proliferation and differentiation of MSCs derived from PDL.

为阐明牙周膜间充质干细胞(MSCs)增殖和分化调控的分子机制，我们建立了两种单细胞来源的大鼠韧带细胞培养体系(SCDC2)和SCDC2(SCDC2)，其中SCDC2具有自我更新和多向分化的能力，SCDC1不具有自我更新和多能性。然后，我们综合比较了SCDC1和SCDC2的基因表达状态和胞内分子的磷酸化状态。我们发现，在54,000个靶基因中，约有600个基因被鉴定为两种类型细胞之间的差异表达基因。特别是，我们发现一些先前被报道为几种干细胞的增殖和分化调节因子的转录因子在SCDC2细胞中高表达。此外，我们还发现，成纤维细胞生长因子和转化生长因子诱导的细胞内信号转导在骨髓间充质干细胞的增殖和分化调控中起着重要的作用。因此，我们成功地阐明了PDL来源的MSCs增殖和分化调控的分子机制。

项目成果

ヒト乳歯歯根膜由来細胞におけるSDF-1の発現調節機構

人乳牙牙周膜来源细胞中SDF-1表达的调控

• 发表时间: 2010
• 作者: 伊東俊太郎;吉田康夫;須和部京介;國松和司;長谷川智一
• 通讯作者: 長谷川智一

システインリッチペプチドSCRG1は間葉系幹細胞の移動能を促進する

富含半胱氨酸的肽SCRG1促进间充质干细胞的迁移能力

• 发表时间: 2010
• 作者: Hayashi Y;Zhang R;Sun L;Wu Z;Nakanishi H.;帖佐直幸
• 通讯作者: 帖佐直幸

線維芽細胞増殖因子(FGF-1)によるラット歯周靭帯由来未分化間葉系細胞様細胞の増殖促進効果発現メカニズム

成纤维细胞生长因子(FGF-1)促进大鼠牙周膜源性未分化间充质细胞样细胞增殖的机制

• 发表时间: 2010
• 期刊: 口腔組織培養学会誌 20
• 作者: 高橋美香子;大久保直登;帖佐直幸;高橋典子;加茂政晴;水城春美;石崎明;客本斉子
• 通讯作者: 客本斉子

FGFとTGF-βスーパーファミリーの相互作用による未分化間葉系幹細胞の細胞増殖・分化制御機構の解明

通过FGF与TGF-β超家族相互作用阐明未分化间充质干细胞的细胞增殖和分化控制机制

• 发表时间: 2009
• 作者: Yukata K;Matsui Y;Shukunami C;Takimoto A;Goto T;Nishizaki Y;Nakamichi Y;Kubo T;Sano T;Kato S;Hiraki Y;Yasui N.;石崎明
• 通讯作者: 石崎明

未分化間葉系細胞の増殖・分化制御メカニズムの解明-歯周靱帯再生医療の実現を目指して-

阐明未分化间充质细胞增殖和分化的控制机制 - 旨在实现牙周膜再生医学 -

• 发表时间: 2011
• 作者: Matsunami M;Tarui T;et al.;西下一久;石崎明
• 通讯作者: 石崎明