Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy

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Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy The aim of this study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacterial identification, before and after root canal treatment. Samples from infected root canals before and after treatment were collected, followed by anaerobic culture. After incubation, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. The findings of this study suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.