Research of inhibitors for nuclear transport or enzyme activity of protein kinases encoded by human herpesviruses

人疱疹病毒编码蛋白激酶核转运或酶活性抑制剂的研究

• 批准号: 20611009
• 负责人: ISEGAWA Yuji
• 金额: $ 2.91万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20611009/
• 关键词: ヘルペスウイルス; GCV耐性ウイルス; 蛋白キナーゼ; 核輸送; 検査法; 阻害剤; HHV-6; DNAポリメラーゼ

Human herpesvirus 6 (HHV-6) ganciclovir-resistant strain was obtained with amino acid substitutions associated with the death of a recipient after an allogeneic stem cell transplant recipient. The substitutions on U38 DNA polymerase of HHV-6 were associated with the ganciclovir-resistance of HHV-6. The assays to detect antiviral drug-resistance mutations of HHV-6 were developed. The assay has been developing the powerful detection system which is rapid and multi-screening method. The nuclear transport mechanism of U69 protein was cleared, and it is started that the multi-screening assay system is been developing to detect the inhibitors for the nuclear transport.

人类疱疹病毒6型(HHV-6)更昔洛韦耐药株是通过与异基因干细胞移植受者死亡相关的氨基酸替换获得的。HHV-6的U38DNA聚合酶的突变与HHV-6对更昔洛韦的耐药性有关。建立了检测HHV-6耐药突变的方法。本检测已发展成为一种快速、多筛查、功能强大的检测系统。阐明了U69蛋白的核转运机制，并开始建立检测核转运抑制物的多筛选检测系统。

项目成果

Human herpesvirus 6 ganciclovir-resistant strain with amino acid substitutions associated with filedeath of a recipient after an allogeneic stem cell transplant recipient

人类疱疹病毒 6 更昔洛韦耐药株，具有与同种异体干细胞移植受者后受者死亡相关的氨基酸取代

• 发表时间: 2009
• 期刊: Journal of Clinical Virology 44
• 作者: Y. Iseeawa;J. Hara;K. Amo;Y. Osugi;M. Takanoto;K. Yamanishi;R. Fukunaga;M. Shibata;A. Ohshima;Y. Horiguchi;N. Sugimoto
• 通讯作者: N. Sugimoto

PCR with quenching probes enables the rapid detection and identification of ganciclovir-resistant causing U69 gene mutations in human herpesvirus 6

使用猝灭探针的 PCR 能够快速检测和鉴定人类疱疹病毒 6 中导致 U69 基因突变的更昔洛韦耐药性

• 发表时间: 2010
• 期刊: Molecular and Cellular Probes 24(In press)
• 作者: Y.Isegawa;C.Matsumoto;K.Nishinaka;K.Nakano;T.Tanaka;N.Sugimoto.A.Ohshima
• 通讯作者: N.Sugimoto.A.Ohshima

U83 gene Variations Among Human Herpesvirus 6 from Exanthem Subitum patients, Transplant Recipients, and Healthy Donors.

来自 Exanthem Subitum 患者、移植受者和健康捐赠者的人类疱疹病毒 6 中的 U83 基因变异。

• 发表时间: 2008
• 作者: R.Sjahril;Y.Isegawa;T.Tanaka;K.Nakano;T.Yoshikawa;Y.Asano;A.Ohshima;K.Yamanishi;N.Sugimoto
• 通讯作者: N.Sugimoto

Human herpesvirus 6 ganciclovir-resistant strain with amino acid substitutions associated with the death of a recipient after an allogeneic stem cell transplant recipient.

人类疱疹病毒 6 更昔洛韦耐药株，具有与同种异体干细胞移植受者后受者死亡相关的氨基酸取代。

• 发表时间: 2009
• 期刊: Journal of Clinical Virology 44
• 作者: Y.Isegawa J.Hara;K.Amo;Y.Osugi;M.Takemoto;K.Yamanishi;R.Fukunaga;M.Shibata;A.Ohshima;Y.Horiguchi;N.Sugimoto
• 通讯作者: N.Sugimoto

Relationship between U83 gene variation in humen herpesvirus 6 and secretion of the U83 gene product

人疱疹病毒6型U83基因变异与U83基因产物分泌的关系

• 发表时间: 2009
• 期刊: Archives of Virology 154
• 作者: R.Sjahril;Y.Isegawa;T.Tanaka;K.Nakano;T.Yoshikawa;Y.Asano;A.Ohshima;K.Yamanishi;N.Sugimoto
• 通讯作者: N.Sugimoto