Development of targeting method of subcellular organelles using focusing heavy-ion microbeam.

为了阐明重离子辐照细胞生物学效应的机制，发展了一种利用聚焦重离子微束靶向细胞亚细胞区域的方法。进行了细胞靶向系统的安装、用于制备靶样品的方案的建立以及可以从荧光染料染色的细胞图像提取细胞坐标的图像分析代码的开发。所开发的系统能够使用聚焦重离子微束靶向和照射单个培养细胞。

To elucidate mechanisms underlying biological responses of heavy-ion irradiated cells, a targeting method of subcellular regions using focusing heavy-ion microbeam was developed. An installation of a cell targeting system, an establishment of protocol for preparing target sample, and a development of an image analysis code that can extract cell coordinates from fluorescent-dye-stained cell image were carried out. The developed system was able to target and irradiate individual cultured cells using focusing heavy-ion microbeam.