Structure determination method for non-crystallizable proteins by synchrotron radiation hard X-rays

同步辐射硬X射线测定非结晶蛋白结构的方法

• 批准号: 20612022
• 负责人: IWAMOTO Hiroyuki
• 金额: $ 2.91万
• 依托单位: Japan Synchrotron Radiation Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20612022/
• 关键词: 量子ビーム; 放射光硬X線; 結晶化困難タンパク質; 流動配向法; X線ホログラフィー

About 70% of currently used medical drugs are said to be targeted to membrane proteins. Because of this, the knowledge about the 3D structure of these proteins is indispensable for the development of new drugs. However, the structures of only a few of the membrane proteins have been solved, because it is generally difficult to obtain crystals of these proteins, which are indispensable for the standard procedure of structure determination. This study is conducted to develop the principles needed to determine the structure of these proteins without making crystals, by applying the technique of X-ray holography.

据说目前使用的医疗药物中约有70%是针对膜蛋白的。正因为如此，了解这些蛋白质的三维结构对于新药的开发是必不可少的。然而，只有少数膜蛋白的结构得到了解决，因为通常很难获得这些蛋白质的晶体，这是确定结构的标准程序所不可缺少的。本研究旨在通过应用x射线全息技术，在不制造晶体的情况下确定这些蛋白质结构所需的原理。

项目成果

Hard X-ray Fourier transform holography from an array of oriented, referenced objects.

来自一系列定向参考物体的硬 X 射线傅立叶变换全息术。

• 发表时间: 2011
• 期刊: J.Synchrotron Rad. 18
• 作者: Iwamoto;H.;Yagi;N.
• 通讯作者: N.

Hard X-ray Fourier transform holography from an array of oriented, referenced objects

来自一系列定向参考物体的硬 X 射线傅里叶变换全息术

• 发表时间: 2011
• 期刊: Journal of Synchrotron Radiation
• 影响因子: 2.5
• 作者: Iwamoto;H.;Yagi;N.
• 通讯作者: N.