Development of nano-imaging method based on molecular charges at cell membrane by use of field effect devices

利用场效应器件开发基于细胞膜分子电荷的纳米成像方法

• 批准号: 20681015
• 负责人: SAKATA Toshiya
• 金额: $ 16.22万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20681015/
• 关键词: 電界効果; 細胞膜; 電荷; プローブ顕微鏡; イメージング; 半導体; ナノバイオ; バイオセンシング

I have developed a biosensing technique to measure molecular charge distribution at cell membrane using a principle of semiconductor. In this research, I have utilized cantilever of atomic force microscopy (AFM) in order to contact with cell membrane and to move electrode at nano scale, and developed "gatelever" with platinum tip to detect charges at cell membrane using AFM. The gatelever as electrode is extended from metal oxide semiconductor (MOS) field effect transistor (FET). Actually, negative charges based on sialic acids and so on at cell membrane was detected using AFM with gatelever-based FET. As a result, membrane protein with some charges at cell membrane can be detected at nano scale using the proposed system.

我已经开发了一种生物传感技术，利用半导体原理测量细胞膜上的分子电荷分布。本研究利用原子力显微镜（AFM）的悬臂梁与细胞膜接触，在纳米尺度上移动电极，并开发了铂针尖的“门控器”，利用AFM检测细胞膜上的电荷。作为电极的栅极从金属氧化物半导体（MOS）场效应晶体管（FET）延伸。实际上，基于唾液酸等在细胞膜上的负电荷使用AFM与基于半导体场效应管的FET检测。结果表明，利用该系统可以在纳米尺度上检测到细胞膜上带有一定电荷的膜蛋白。

项目成果

Development of cell/transistor interface for real-time and noninvasive monitoring of potassium ion release based on apoptosis using biologically-coupled field effect transistor

开发细胞/晶体管接口，使用生物耦合场效应晶体管实时、无创地监测基于细胞凋亡的钾离子释放

• 发表时间: 2010
• 期刊: Transactions of the Materials Research Society of Japan 35
• 作者: Taiichiro Murakami;Toshiya Sakata;Akira Matsumoto;Madoka Takai;Kazuhiko Ishihara;Yuji Miyahara
• 通讯作者: Yuji Miyahara

Open sandwich-based immuno-transistor for label-free and highly sensitive detection of low molecular weight antigen

基于开放夹心的免疫晶体管，用于低分子量抗原的无标记和高灵敏度检测

• 发表时间: 2009
• 期刊: Analytical Chemistry 81
• 作者: Toshiya Sakata;Masaki Ihara;Izumi Makino;Yuji Miyahara;Hiroshi Ueda
• 通讯作者: Hiroshi Ueda

Electrical Detection Ovum Membrane Charges Using Biotransistor

使用生物晶体管电检测卵膜电荷

• 发表时间: 2008
• 期刊: Microelectronics Engineering (In press)
• 作者: Toshiya Sakata;Izumi Makino;Sayaka Kita and Yuji Miyahara
• 通讯作者: Sayaka Kita and Yuji Miyahara

細胞測定装置

电池测量装置

• 发表时间: 2010

Continuous Monitoring of Electrical Activity of Pancreatic β-Cells Using Cell-Based Field Effect Transistor

使用基于细胞的场效应晶体管连续监测胰腺 β 细胞的电活动

• 发表时间: 2011
• 期刊: Japanese Journal of Applied Physics
• 影响因子: 1.5
• 作者: Toshiya Sakata;Haruyo Sugimoto
• 通讯作者: Haruyo Sugimoto