Development of nano-imaging method based on molecular charges at cell membrane by use of field effect devices

我开发了一种利用半导体原理测量细胞膜上分子电荷分布的生物传感技术。在这项研究中，我利用原子力显微镜（AFM）的悬臂梁与细胞膜接触，在纳米尺度上移动电极，并开发了带有铂尖的“门式探针”，利用AFM检测细胞膜上的电荷。栅极作为电极是由金属氧化物半导体（MOS）场效应晶体管（FET）扩展而来的。实际上，利用基于栅极效应管的原子力显微镜可以检测细胞膜上基于唾液酸等的负电荷。因此，利用该系统可以在纳米尺度上检测细胞膜上带电荷的膜蛋白。

I have developed a biosensing technique to measure molecular charge distribution at cell membrane using a principle of semiconductor. In this research, I have utilized cantilever of atomic force microscopy (AFM) in order to contact with cell membrane and to move electrode at nano scale, and developed "gatelever" with platinum tip to detect charges at cell membrane using AFM. The gatelever as electrode is extended from metal oxide semiconductor (MOS) field effect transistor (FET). Actually, negative charges based on sialic acids and so on at cell membrane was detected using AFM with gatelever-based FET. As a result, membrane protein with some charges at cell membrane can be detected at nano scale using the proposed system.