Development of Detection Method of Sporadic Viruses from Wastewater

检查废水样本可能是了解病毒实际流行和流行病学的有效方法，因为废水中含有所有人群散发的病毒，无论症状如何。本研究建立了污水中诺如病毒和艾奇病毒的检测方法，并对污水中诺如病毒和艾奇病毒的流行情况和遗传多样性进行了研究，采用RT-PCR方法从污水中检测到大量诺如病毒，并通过克隆测序方法成功地测定了诺如病毒的核酸序列。诺如病毒GI比诺如病毒GII更频繁和丰富地从河水样品中发现。这一结果表明，诺如病毒GI比流行病学结果预期的更流行。我们也用类似的方法检测了艾奇病毒，发现其多样性比预期的要高。

Examining wastewater samples could be an effective approach to understand the actual prevalence and epidemiology of the viruses, since wastewater contains viruses shed from all populations, regardless of symptoms. In this study, we developed methodology and investigated the prevalence and genetic diversity of Norovirus and Aichivirus in wastewater.Noroviruses were found abundantly in wastewater samples by RT-PCR methods, from which we succeeded in determining nucleic acid sequences by the cloning and sequencing method. Norovirus GI was more frequently and abundantly found from river water samples than Norovirus GII. This result implies that Norovirus GI was more popular than expected from epidemiological result. Aichivirus was also tested with similar method and revealed to be more diverse than expected previously.In conclusion, we successfully detected targeted viruses from wastewater.