Development of Detection Method of Sporadic Viruses from Wastewater

废水中散发病毒检测方法的研制

• 批准号: 20686035
• 负责人: KATAYAMA Hiroyuki
• 金额: $ 16.22万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20686035/
• 关键词: 用排水システム; ヒト腸管系ウイルス; 病原微生物; 都市水循環; 安全管理; 下水処理; 遺伝子工学

Examining wastewater samples could be an effective approach to understand the actual prevalence and epidemiology of the viruses, since wastewater contains viruses shed from all populations, regardless of symptoms. In this study, we developed methodology and investigated the prevalence and genetic diversity of Norovirus and Aichivirus in wastewater.Noroviruses were found abundantly in wastewater samples by RT-PCR methods, from which we succeeded in determining nucleic acid sequences by the cloning and sequencing method. Norovirus GI was more frequently and abundantly found from river water samples than Norovirus GII. This result implies that Norovirus GI was more popular than expected from epidemiological result. Aichivirus was also tested with similar method and revealed to be more diverse than expected previously.In conclusion, we successfully detected targeted viruses from wastewater.

检查废水样本可能是了解病毒实际流行和流行病学的有效方法，因为废水中含有所有人群散发的病毒，无论症状如何。本研究建立了污水中诺如病毒和艾奇病毒的检测方法，并对污水中诺如病毒和艾奇病毒的流行情况和遗传多样性进行了研究，采用RT-PCR方法从污水中检测到大量诺如病毒，并通过克隆测序方法成功地测定了诺如病毒的核酸序列。诺如病毒GI比诺如病毒GII更频繁和丰富地从河水样品中发现。这一结果表明，诺如病毒GI比流行病学结果预期的更流行。我们也用类似的方法检测了艾奇病毒，发现其多样性比预期的要高。

项目成果

First detection of genotype 3 hepatitis E virus RNA in river water in Cambodia.

柬埔寨首次在河水中检测到基因 3 型戊型肝炎病毒 RNA。

• 发表时间: 2009
• 期刊: Transactions of the Royal Society of Tropical Medicine and Hygiene. 103(9)
• 作者: Masaaki Kitajima;Koichi Matsubara;Sethy Sour;Eiji Haramoto;Hiroyuki Katayama;Shinichiro Ohgaki.
• 通讯作者: Shinichiro Ohgaki.

Real-time RT-PCRによるアイチウイルスの核酸検出および遺伝子型識別法の開発

开发使用实时RT-PCR的爱知病毒核酸检测和基因分型方法

• 发表时间: 2009
• 作者: 端昭彦;北島正章;山下照夫;皆川洋子;片山浩之;大垣眞一郎
• 通讯作者: 大垣眞一郎

Genetic diversity of genogroup IV noroviruses in wastewater in Japan.

日本废水中基因组 IV 诺如病毒的遗传多样性。

• 发表时间: 2010
• 期刊: Letters in Applied Microbiology Vol.52, Issue 2
• 作者: Masaaki Kitajima;Tomoichiro Oka;Eiji Haramoto;Chanetta Phanuwan;Naokazu Takeda;Kazuhiko Katayama;Hiroyuki Katayama.
• 通讯作者: Hiroyuki Katayama.

Genetic diversity of genogroup IV noroviruses in wastewater in Japan

日本废水中基因组 IV 诺如病毒的遗传多样性

• 发表时间: 2010
• 期刊: Letters in Applied Microbiology
• 影响因子: 2.4
• 作者: M Kitajima;T Oka;E Haramoto;C Phanuwan;N Takeda;K Katayama;H Katayama
• 通讯作者: H Katayama

Seasonal distribution and genetic diversity of genogroups I, II, and IV noroviruses in the Tamagawa River

玉川I、II、IV基因组诺如病毒的季节性分布和遗传多样性

• 发表时间: 2010
• 期刊: Japan.Environmental Science & Technology. 44(18)
• 作者: Masaaki Kitajima;Tomoichiro Oka;Eiji Haramoto;Naokazu Takeda;Kazuhiko Katayama;Hiroyuki Katayama
• 通讯作者: Hiroyuki Katayama