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耳石の微量元素組成と安定同位体組成を指標とした魚類の回遊と集団構造の解析

以耳石微量元素组成和稳定同位素组成为指标分析鱼类洄游和种群结构

基本信息

批准号：
20688008
负责人：
新井崇臣
金额：
$ 14.81万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (A)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20688008/
关键词：
耳石微量元素系群判別回遊履歴 LA-ICPMS EPMA 回遊魚多様性ニシン通し回遊魚

项目摘要

本年度は、レーザーアブレーション高周波誘導結合プラズマ質量分析法や波長分散型X線分析装置を用いて魚類耳石中の微量元素を多元種同時に測定し、水産重要種であるニシンやウナギなどの回遊魚の解析を行った。ニシンの飼育は、水温一定(13℃)で塩分を変化させた3つの実験群(11,23,35‰)、塩分一定(23‰)で水温を変化させた3つの実験群(9,13,17℃)で行なった。ニシンは、孵化から60日間飼育し、これを標本とした。耳石中の微量元素組成(Na/Ca,Mg/Ca,K/Ca,Sr/Ca,Ba/Ca)は、レーザーアブレーション誘導結合プラズマ質量分析法(LA-ICP-MS)を用いて測定した。得られた耳石中の元素組成と、水温および塩分との関係性は、分散分析法および相関係数を用いて解析した。水温を変化させた実験群間においては、測定した全ての元素カルシウム比において有意差が見出された。一方、塩分を変化させた実験群間においては、K/Ca、Sr/CaおよびBa/Ca比で有意な差が見出された。特に、Sr/Ca比と塩分は、強い正の相関関係を示した。以上の結果から、ニシン耳石中の元素組成は、水温と塩分から影響を受けるが、その影響の程度は元素によって異なることが示唆された。インドネシアジャワ島で採集された熱帯ウナギA.bicolor bicolorを用いて回遊履歴に推定を行ったところ、4つの回遊型が見いだされた。このことから、インドネシアに生息する本種は、多様な回遊生態を有することが明らかになった。

This year, combined with the method of wave length dispersive X-ray analysis, this year's high-frequency high-cycle wave analysis has been used for simultaneous determination of trace elements in common otolith, and for the simultaneous determination of trace elements in otolith, and for the determination of trace elements in water samples. When the water temperature is constant (13 ℃), the water temperature is fixed (13 ℃), the water temperature is fixed (13 ℃), the water temperature is constant (13 ℃), the water temperature is fixed (23 ‰), the water temperature is fixed (23 ‰), and the temperature is constant (23 ‰). In the 60-day period of incubation and incubation, the standard and standard of the incubator shall be registered. The content of trace elements in otolith was determined by the combination of trace element composition (Na/Ca,Mg/Ca,K/Ca,Sr/Ca,Ba/Ca) and quantitative analysis (LA-ICP-MS). The composition of the elements in the otolith, the temperature of the water, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, the temperature of the water, the composition of the elements in the otolith, the temperature of the water, The temperature of the water is different from each other, and the elements of the whole system are measured. The temperature of the water is different from that of the whole group. On the one hand, customers are interested in sending customers, K/Ca, and Sr/Ca customers to each other, and their Ba/Ca is intentionally different from each other. Special, Sr/Ca comparison score, strong and positive phase display. The above results show that the composition of the elements in the otolith, the temperature of the water, the degree of the temperature, the temperature, the degree, the degree, the temperature, the temperature, the In this case, you can use the presumption that the line is not valid, and the 4-fold response is in the form of an A.bicolor bicolor. The information system, the information system, and the information system are available in the current and multi-source ecological systems.