Novel neuronal network underlying Drosophila olfactory learning and memory

果蝇嗅觉学习和记忆的新型神经元网络

• 批准号: 20700283
• 负责人: MURAKAMI Satoshi
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20700283/
• 关键词: 分子; 細胞神経科学; 嗅覚記憶; ショウジョウバエ; 条件付け自動化; アルコールセンサー; 神経回路; 記憶の固定; キノコ体接続神経; Labview; JNK

Drosophila olfactory aversive conditioning has served as a powerful model system with which to elucidate the molecular and neuronal mechanisms underlying memory formation. I have devised a simple, low-cost semi-automated conditioning apparatus that computationally controls the delivery of odor and shock. The system is applicable to the study of olfactory memory formation and to the examination of conditioning parameters at a level of detail not practical with a manual apparatus.To identify a novel neuronal network underlying Drosophila olfactory memory, I conducted a Gal4 enhancer-trap screening and identified a pair of neurons innervating onto the mushroom body (MB) in which olfactory memory is encoded. Transient silencing of synaptic transmissions from identified neurons, we call them PMO neurons, with Shibire^<ts> showed that these neurons are specifically involved in the formation of the late stage of (3 hr) olfactory aversive memory. They were not required for the early stage (1 hr) of olfactory memory. These data imply the presence of a neuronal circuit for memory consolidation that function downstream of the MB.

果蝇的嗅觉厌恶条件反射已经成为一个强有力的模型系统，用来阐明记忆形成的分子和神经机制。我设计了一个简单的，低成本的半自动调节装置，它通过计算控制气味和震动的传递。该系统适用于研究嗅觉记忆的形成和检查的条件反射参数的详细程度不实用的手动apparent.To确定一种新的神经元网络的果蝇嗅觉memory.To的基础上，我进行了Gal4增强陷阱筛选，并确定了一对神经元支配的蘑菇体（MB）中的嗅觉记忆编码。来自已识别神经元（我们称其为PMO神经元）的突触传递的短暂沉默，Shibire^<ts>表明这些神经元特别参与（3小时）嗅觉厌恶记忆后期的形成。嗅觉记忆的早期阶段（1小时）不需要它们。这些数据意味着存在一个用于记忆巩固的神经元回路，其功能在MB下游。

项目成果

Identification of a mushroom body output neuron required during the consolidation phase for odor memory

识别气味记忆巩固阶段所需的蘑菇体输出神经元

• 发表时间: 2010
• 作者: Satoshi Murakami;Kohei Sekiguchi;Toshiharu Ichinose;Yuko Maeyama;Tetsuya Tabata;村上智史
• 通讯作者: 村上智史

Transcriptome analysis of olfactory aversive memory in Drosophila mushroom body

果蝇蘑菇体嗅觉厌恶记忆的转录组分析

• 发表时间: 2009
• 作者: Satoshi Murakami;Yuko Maeyama;Tetsuya Tabata
• 通讯作者: Tetsuya Tabata

Evaluaing the stimuli coincidence in aversive olfactory learning of Drosopli Ha with a semi-automated apparatus

用半自动装置评估果蝇 Ha 厌恶性嗅觉学习中的刺激一致性

• 发表时间: 2008
• 作者: Satoshi Murakami;Yuko Maeyama;Tetsuya Tabata;村上智史;村上智史;村上智史;村上智史;村上智史
• 通讯作者: 村上智史

Identification of a mushroom body output neuron required during the consolidation phase for odor memory匂い記憶の固定期に働くキノコ体出力神経の同定

识别气味记忆巩固阶段所需的蘑菇体输出神经元

• 发表时间: 2010
• 作者: Satoshi Murakami;Kohei Sekiguchi;Toshiharu Ichinose;Yuko Maeyama;Tetsuya Tabata
• 通讯作者: Tetsuya Tabata

Transcriptome analysis of olfactory averaive memory in Drosophila mushroom body

果蝇蘑菇体嗅觉平均记忆的转录组分析

• 发表时间: 2009
• 作者: Satoshi Murakami;Yuko Maeyama;Tetsuya Tabata;村上智史
• 通讯作者: 村上智史