Analysis of mouse higher-order visual cortex by differential imaging

差分成像分析小鼠高阶视觉皮层

• 批准号: 20700347
• 负责人: TOHMI Manavu
• 金额: $ 2.83万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20700347/
• 关键词: 高次視覚領域; フラビン蛍光イメージング; 差分イメージング; 経験依存的可塑性; in vivo; プロトカドヘリン; ノックアウトマウス; スライス

It became possible to separate and to identify mouse's several higher-order visual areas by the combination with the pattern stimulus having different speed with flavin fluorescent imaging. Therefore the high order visual areas which were difficult to study until that time were observed. The properties of neurons in each area were different in the directional selectivity and the preferred stimulation speed. The property was disturbed in the animal that had bred under the flash light condition. Moreover, the anatomical combinations of each region were observed using with neural traser. Finally, abnormality was discovered in the property of the higher-order visual areas in the knockout mouse of cell-adhesion molecule CNR.

利用不同速度的图形刺激与黄素荧光成像相结合，可以分离和识别小鼠的几个高阶视觉区。因此，观察到了在此之前难以研究的高阶视觉区域。不同脑区神经元的特性在方向选择性和偏好刺激速度上存在差异。在闪光灯条件下繁殖的动物中，该特性受到干扰。并应用神经示踪仪观察各区域的解剖组合。最后，在细胞粘附分子CNR基因敲除小鼠中发现了高阶视觉区性质的异常。

项目成果

Transcranial flavoprotein fluorescence imaging of mouse cortical activity and plasticity

小鼠皮质活动和可塑性的经颅黄素蛋白荧光成像

• 发表时间: 2009
• 期刊: J Neurochem 109Suppl.1
• 作者: Tohmi M;Takahashi K;Kubota Y;Hishida R;Shibuki K
• 通讯作者: Shibuki K

Autofluorescence imaging revealed the property and the plasticity of the mouse high order visual cortex

自发荧光成像揭示了小鼠高阶视觉皮层的特性和可塑性

• 发表时间: 2008
• 作者: M Tohmi;R Meguro;R Hishida;M Norita;T Yagi;K Shibuki;M. Tohmi;M. Tohmi;M. Tohmi
• 通讯作者: M. Tohmi

Imaging of higher visual cortices based on differences in response properties between the primary and higher visual cortices in mice

基于小鼠初级视觉皮层和高级视觉皮层之间反应特性的差异对高级视觉皮层进行成像

• 发表时间: 2009
• 作者: M.Tohmi;K.Takahashi;Y.Kubota;R.Hishida;K.Shibuki;M. Tohmi
• 通讯作者: M. Tohmi

The Autofluorescence imaging revealed the property and the plasticity of the mouse high order visual cortex

自发荧光成像揭示了小鼠高阶视觉皮层的特性和可塑性

• 发表时间: 2008
• 作者: M Tohmi;R Meguro;R Hishida;M Norita;T Yagi;K Shibuki;M. Tohmi;M. Tohmi;M. Tohmi;M. Tohmi
• 通讯作者: M. Tohmi

In In vivo optical imaging of brain function 2nd Edition

脑功能体内光学成像第二版

• 发表时间: 2009
• 作者: K. Shibuki;R. Hishida;M. Tohmi;K. Takahashi;H. Kitaura;Y. Kubota
• 通讯作者: Y. Kubota