Search for fluorescent probe for high sensitive immunnosensing by Surface Plasmon Field-enhanced Fluorescence Spectroscopy

1)对可见光表面等离子体场增强荧光测量装置进行改进，构建了近红外表面等离子体场增强荧光光谱（SPFS）测量装置。2)采用近红外SPFS快速定量检测肿瘤标志物（AFP； α胎蛋白）。等离子体样品可在15分钟内测得，比可见SPFS提高约20倍，检出限为0.005 ng/mL。3)寻找近红外荧光探针。单壁碳纳米管（SWNT）在传感器表面的非特异性吸附无法控制，也没有达到特征的光谱评价。通过与传感器表面（金薄膜表面）取适当的间隔距离，在使用有机染料时具有很高的灵敏度。

1) The device of near-infrared Suface Plasmon field-enhanced Fluorescence Spectroscopy (SPFS) measurement was constructed by the visible optical suface plasmon field-enhanced fluorescence measurement device improvement.2) The fast quantification of the tumor marker (AFP ; alpha fetoprotein) with near-infrared SPFS was done. The plasma sample would be measured in 15 minutes, it improved by about 20 times compared with the visible SPFS and the limit of detection was 0.005 ng/mL.3) It searched for the near-infrared fluorescent probe. The nonspecific adsorption of single-walled carbon nanotube (SWNT) to the sensor surface could not be controlled, and it did not arrive at the spectrum evaluation of characteristics. By taking the appropriate distance of the interval with the sensor surface (surface of gold thin film), it was very high sensitivity when the organic dye was used.