Search for fluorescent probe for high sensitive immunnosensing by Surface Plasmon Field-enhanced Fluorescence Spectroscopy

1)利用可见光表面等离子体激元增强荧光测量装置构建了近红外表面等离子体增强荧光光谱(SPFS)测量装置。2)实现了近红外SPFS对肿瘤标志物甲胎蛋白(AFP)的快速定量。血浆样品的测量时间为15分钟，比可见光谱分析提高了约20倍，检测下限为0.005 ng/mL.3)，寻找了近红外荧光探针。单壁碳纳米管(SWNT)在传感器表面的非特异性吸附无法控制，没有达到光谱特性的评价。通过取与传感器表面(金膜表面)的适当距离，当使用有机染料时，灵敏度很高。

1) The device of near-infrared Suface Plasmon field-enhanced Fluorescence Spectroscopy (SPFS) measurement was constructed by the visible optical suface plasmon field-enhanced fluorescence measurement device improvement.2) The fast quantification of the tumor marker (AFP ; alpha fetoprotein) with near-infrared SPFS was done. The plasma sample would be measured in 15 minutes, it improved by about 20 times compared with the visible SPFS and the limit of detection was 0.005 ng/mL.3) It searched for the near-infrared fluorescent probe. The nonspecific adsorption of single-walled carbon nanotube (SWNT) to the sensor surface could not be controlled, and it did not arrive at the spectrum evaluation of characteristics. By taking the appropriate distance of the interval with the sensor surface (surface of gold thin film), it was very high sensitivity when the organic dye was used.