Microwave-assisted rapid fabrication of high performance monolithic capillary column

采用微波辐射法快速制备了硅胶基和聚合物基毛细管整体柱。将反应溶液填充到熔融石英毛细管中，然后在不同温度和微波装置的输出控制下在不同时间照射柱。还用扫描电子显微镜（SEM）观察整料的形态，并与在常规条件下制造的那些整料进行比较。结果表明，对于硅基整体柱，在溶胶-凝胶反应过程中，以300 W的功率辐照1 h，可获得高性能的毛细管柱，而溶胶-凝胶反应通常进行24 h。对于聚合物基整体柱，在200 W下辐射输出15 min足以制备用于常规液相色谱分离的相对高性能毛细管柱。

Rapid fabrication of monolithic silica- and polymer-based capillary columns were evaluated using microwave irradiation. The reaction solution was filled into fused-silica capillary tubing and the column was then irradiated at different time under various temperature and output control of the microwave device. The morphology of the monoliths was also observed with a scanning electron microscope (SEM) and was compared to those monoliths that were fabricated under conventional conditions. As the results, as for the silica-based monolith, high performance capillary column was obtained when the column was irradiated at output 300 W for 1 h during the sol-gel reaction, which was normally carried out for 24 h. As for the polymer-based monolith, radiation output at 200W for 15 min was enough to make a relatively high performance capillary column for normal liquid chromatographic separation.