Microwave-assisted rapid fabrication of high performance monolithic capillary column

研究了微波辐照快速制备整体硅基和聚合物基毛细管柱的方法。将反应溶液填充到熔融石英毛细管中，然后在微波装置的不同温度和输出控制下，在不同时间照射色谱柱。用扫描电子显微镜（SEM）观察了单块石的形貌，并与在常规条件下制备的单块石进行了比较。结果表明，对于硅基单体柱，在溶胶-凝胶反应过程中，以300w输出功率照射1 h，获得了高性能的毛细管柱，而通常的反应时间为24h。对于聚合物基单体柱，200W输出功率照射15 min，足以形成一个相对高性能的毛细管柱，用于正常的液相色谱分离。

Rapid fabrication of monolithic silica- and polymer-based capillary columns were evaluated using microwave irradiation. The reaction solution was filled into fused-silica capillary tubing and the column was then irradiated at different time under various temperature and output control of the microwave device. The morphology of the monoliths was also observed with a scanning electron microscope (SEM) and was compared to those monoliths that were fabricated under conventional conditions. As the results, as for the silica-based monolith, high performance capillary column was obtained when the column was irradiated at output 300 W for 1 h during the sol-gel reaction, which was normally carried out for 24 h. As for the polymer-based monolith, radiation output at 200W for 15 min was enough to make a relatively high performance capillary column for normal liquid chromatographic separation.