PET and fluorescence imaging by using novel protein modification techniques

我们为肽和蛋白质的n端特异性荧光标记和PET标记提供了一种新的方法。此外，我们将L/ f转移酶与大肠杆菌苯丙烯酰trna合成酶（ARS）结合，实现了非核糖体n端特异性引入多种非天然氨基酸到蛋白质上。一种非天然氨基酸一旦被突变的ARS原位加载到大肠杆菌tRNAPhe上，并依次从tRNA转移到靶蛋白上，即NEXT-A反应。

We provided a novel method for N-terminal specific fluorescence labeling and PET labeling of peptide and proteins.Moreover, combining L/F-transferase with E. coli phenylalanyl-tRNA synthetase (ARS), we achieved non-ribosomal N-terminal-specific introduction of various kinds of nonnatural amino acids to a protein. A nonnatural amino acid is once charged onto an E. coli tRNAPhe by a mutant ARS in situ, and successively transferred from the tRNA to a target protein, namely the NEXT-A reaction.