Nonlinear microspectroscopic imaging by use of few-cycle optical pulses

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We have developed nonlinear optical microscopy by use of ultra-short laser pulses with a duration of <8 fs. Specifically, we succeeded in the group-velocity dispersion compensation with chirp-mirrors and a spatial light modulator to enhance the sensitivity of stimulated parametric emission (SPE) microscopy by 28dB. We observed various samples with this system, and found that red blood cells and petal cells exhibit strong SPE contrast. Furthermore, we came up to the idea of stimulated Raman scattering (SRS) microscopy, confirmed its principle, and improved its sensitivity. These results suggest successful development of powerful biological imaging tools by use of ultra-short laser pulses.