Fundamental study for the cultural development of anaerobic oxidation of methane with denitrification.

甲烷厌氧氧化反硝化培养发展的基础研究。

• 批准号: 20760357
• 负责人: ITO Tsukasa
• 金额: $ 2.75万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20760357/
• 关键词: メタン酸化; メタン脱窒; 環境技術; 地球温暖化ガス排出削減; 水質汚濁・土壌汚染防止・浄化; 脱窒; 嫌気メタン酸化; メタン; 応用微生物; 環境

The significance of anaerobic oxidation of methane with denitrification in fresh water habitats is largely unknown. First, we tried to enrich the microbial community that denitrify with methane and nitrate under anaerobic condition. A portion of a river sediment and anaerobic methanogenic granular sludge were inoculated for the culture. High denitrifying activity was observed in the anaerobic granular sludge. However, the denitrification rate was still too low to investigate what parameters influence the methane denitrifying activity. Therefore, we developed the bioreactor which increased gas concentration such as methane in the liquid culture medium. The developed bioreactor was able to distribute microbubbles in the water phase and to keep high concentration of gas such as oxygen and methane.

淡水生境中甲烷厌氧氧化和反硝化的意义在很大程度上是未知的。首先，我们试图丰富在厌氧条件下用甲烷和硝酸盐进行反硝化的微生物群落。接种部分河流底泥和厌氧产甲烷颗粒污泥进行培养。厌氧颗粒污泥具有较高的反硝化活性。然而，反硝化速率仍然很低，无法研究哪些参数对甲烷反硝化活性的影响。为此，我们研制了提高液体培养基中甲烷等气体浓度的生物反应器。所开发的生物反应器能够在水相中分布微泡，并保持较高的氧气和甲烷等气体浓度。

项目成果

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