Fundamental study for the cultural development of anaerobic oxidation of methane with denitrification.

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The significance of anaerobic oxidation of methane with denitrification in fresh water habitats is largely unknown. First, we tried to enrich the microbial community that denitrify with methane and nitrate under anaerobic condition. A portion of a river sediment and anaerobic methanogenic granular sludge were inoculated for the culture. High denitrifying activity was observed in the anaerobic granular sludge. However, the denitrification rate was still too low to investigate what parameters influence the methane denitrifying activity. Therefore, we developed the bioreactor which increased gas concentration such as methane in the liquid culture medium. The developed bioreactor was able to distribute microbubbles in the water phase and to keep high concentration of gas such as oxygen and methane.