Sequence-specific separation of functional nucleic acids by liquid-liquid extraction using molecular nano-selfassembly

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We developed DNA-surfactant as an extractant for sequence-specific DNA extraction in a reverse-micellar system. The DNA-surfactant is a short DNA oligonucleotide conjugated with a hydrophobic oleoyl group. A DNA-surfactant is selectively hybridized to a complementary target DNA to form a double helix. The helix is transferred into a water-pool of a reverse micelle, resulting in the DNA extraction to an organic phase. As a result, we achieved sequence-selective DNA extraction.