Development of molecular target peptide screening system by using a highly effective protein immobilization

我们研究了一种新的肽药物筛选系统，通过该系统，可以有效地获得与FoxP3（靶蛋白）功能结构域结合的亲和肽，通过应用我们小组开发的高功能蛋白固定化方法。初步验证了剪切FoxP3的功能性固定化。另外，从T7噬菌体随机肽库中筛选到了几个能显著抑制FoxP3功能区与相关寡核苷酸结合的亲和肽。

We have examined a novel peptide drug screening system by which the affinity peptides that bind to functional domain of FoxP3 (target protein) could be effectively obtained by applying highly functional protein immobilization method developed in our group. As the results, functional immobilization of trimmed FoxP3 was verified at first. In addition, several affinity peptides that could significantly inhibit the binding of relevant oligo DNA to functional domain of FoxP3 were successfully isolated from T7 phage random peptide library.