Development of the method for measurement of cellular dynamics by using single cell time course analysis.

在这项研究中，我们通过测量EGFP（或Venus）转染的HeLa细胞的时间过程，阐明了细胞分裂与基因表达开始之间的强烈相关性。接下来，我们将从3个方面考察这一现象的普遍性：1 .使用9种转染试剂考察转染方法的依赖性；2 .使用HeLa et- on考察转染基因活性的时间延迟对相关性的影响；3 .电穿孔作为物理转染方法对相关性的影响。这些结果表明，细胞核跨核膜转移是基因表达的限速步骤。利用这种方法，我们通过比较细胞分裂的时间和基因表达的开始时间来研究启动子的活性。结果表明，不同的启动子对时间的影响具有统计学意义。我们的方法可以通过量化启动子的活性来测量细胞动力学。

In this study, we elucidate the strong correlation between cell division and onset of gene expression by measuring their time course of HeLa cells, which were taransfected with EGFP (or Venus). In the next, we investigate the generality of this phenomenon by 3 approacches: 1 the dependency of the transfection method by using 9 transfection reagents, 2 the influence of the time delay of the activity of transfected gene on the correlation by using HeLa Tet-On, 3 the influence of the electroporation as the physical transfection method on the correlation. All results suggested that the nuclear transfer across the nuclear membrane is the rate limiting step o gene expression. By using this method, we investigate the activity of promoters by comparing the time period of cell division and onset of gene expression. As the result, the time period had statistically significance depend on the promoter. Our method is able to measure the cellular dynamics by quantify the activity of promoter.