Investigation of a new treatment for implantation failure patients using peripheral monocytes

子宫内膜经历了剧烈的结构和功能变化，使胚胎能够在怀孕早期附着和植入。在小鼠中，巨噬细胞通过暴露于精浆而被招募到子宫内膜，这些细胞似乎能够影响局部细胞通讯和组织重塑事件，从而促进子宫对胚胎着床的接受性。我们前期的研究表明，U937巨噬细胞通过liff介导的STAT3信号传导调节人子宫内膜细胞中胚胎粘附分子的表达。在本研究中，我们利用外周血单核细胞（PBMC）和石川细胞组成的体外模型进一步探讨巨噬细胞-子宫上皮细胞通讯的调节。月经周期中卵巢类固醇激素暴露以及男性精浆改变了PBMC的信号特性，这些因素影响了PBMC调节子宫上皮细胞粘附分子表达的能力。

The uterine endometrium undergoes dramatic structural and functional changes to allow the embryo to attach and implant in early pregnancy. In mice, macrophages are recruited into the endometrium by exposure to seminal plasma and these cells appear able to influence local cell communication and tissue remodeling events to promote uterine receptivity for embryo implantation. Our previous study showed that U937 macrophages regulate embryo adhesion molecule expression in human endometrial cells via LIF-mediated STAT3 signalling. In this study, we utilized an in vitro model comprising peripheral blood monocytes (PBMC) and Ishikawa cells to further explore regulation of macrophage-uterine epithelial cell communication. The signaling characteristics of PBMCs are changed by exposure to ovarian steroid hormones in the menstrual cycle, as well as by male seminal plasma, and that these factors influence PBMC capacity to regulate uterine epithelial cell adhesion molecule expression.