Tissue Engineering for Bio-engineered Trachea

本研究旨在探索加速人工气管内腔表面上皮形成的方法，该方法已应用于气管或喉的外科重建。本研究以成纤维细胞、上皮细胞和人工气管为材料，制备了几种生物工程气管。与气管成纤维细胞相比，异位真皮成纤维细胞在可及性方面是有用的。用生物工程气管重建大鼠气管和喉部缺损后，观察到上皮快速成熟和分化。

The purpose of this study is to search the way to accelerate epithelization on surface of inner lumen of an artificial trachea, which had been clinically applied to surgical reconstructions of trachea or larynx. In this study, we developed several kind of bio-engineered trachea consist of fibroblasts, epithelial cells and the artificial trachea. Heterotopic dermal fibroblasts were useful for those accessibility compared to tracheal fibroblasts. After reconstruction of defects of rats' trachea and larynx with bio-engineered trachea, rapid maturity and differentiation of epithelium were observed.