Study about establishment of control method of novel reovirus presenting a serious acute respiratory symptom

我们从日本的一名男性患者中分离到一种以前未知的呼肠孤病毒（命名为“宫崎病毒”），该患者在病毒分离时患有高烧和急性呼吸道疾病。系统进化树分析表明，该分离株属于纳尔逊湾病毒群，与同时从高热和急性呼吸道感染患者中分离的HK 23629/07有较近的亲缘关系。建立了检测宫崎病毒的RT-PCR和定量RT-PCR方法，检测宫崎病毒抗体的病毒中和试验和IFA、ELISA方法。在实验感染中，我们证明了宫崎病毒引起肺部病毒性肺炎的发病机制。

We isolated a previously unknown reovirus (named 'Miyazaki virus") isolated from a male patient in Japan, who was suffering from high fever and acute respiratory disease at the time of virus isolation. Phylogenetic analysis of the S segment indicated that the isolate belonged to Nelson Bay virus group and was closely related to HK23629/07, isolated from high fever and acute respiratory patient at same time. We developed RT-PCR and quantitative RT-PCR for detection Miyazaki virus, and virus neutralization test and IFA, ELISA for detection of antibodies against Miyazaki virus. In experimental infection, we demonstrated that Miyazaki virus caused pathogenesis of viral pneumonia in the lungs.