Large DNA molecule introduction into animal, plant and yeast cells using Type IV secretion system

IV型分泌系统(T4SS)是一种新型的基因导入真核细胞的方法，它来源于IncP型质粒。我们发现线粒体呼吸突变体在酿酒酵母中表现出更高的转化效率。通过使用线粒体功能的抑制剂，我们成功地建立了一种简单有效的从大肠杆菌中导入酿酒酵母的方法。此外，我们还首次观察到利用该T4SS将基因从大肠杆菌直接转移到高等植物细胞中。

One of Type IV secretion systems (T4SS), which is derived from an IncP type plasmid, was applied as a novel gene introduction method into eukaryotic cells. We found that mitochondrial respiratory mutants in S. cerevisiae showed enhanced transformation efficiency. By using inhibitors of mitochondrial function, we successfully established a simple and efficient gene introduction method into S. cerevisiae from E. coli. In addition, we firstly observed direct gene transfer from E.coli to higher plant cells by applying this T4SS.