Development of DNA-interstrand cross-linking compounds and application to DNA scaffold

我们已经发现，含有双（氨氧基）基团的单个分子能够连接两个AP位点，这是在双链寡核苷酸的“互补”位置上产生的。具有两个氨氧基的芳族分子显示出比具有直链烷基链的交联剂高得多的缀合效率。链间交联（ICL）沿着芳香残基的堆积作用可以稳定ICL双链体。我们在ICL-DNA上进行了两种酶促反应，并表明它们有潜力成为稳定的纳米支架。

We have found that a single molecule containing bis(aminooxy) groups is capable of linking two AP sites, which are produced on "complementary" positions of double-stranded oligonucleotides. The aromatic molecule having two aminooxy groups showed much higher conjugation efficiency than the cross-linker with a straight alkyl chain. The interstrand cross-link(ICL) along with the stacking effect of the aromatic residue could stabilize the ICL duplex. We conducted two kinds of enzymatic reactions on the ICL-DNAs and showed that they have the potential to become stable nanosized scaffolds.