Development of novel glucose sensing capsules with a function of signal enhancement

为了开发一种可用于在体间质液中葡萄糖浓度监测的微创传感器系统，研制了一种基于酶产物跨膜转运调节信号增强功能的葡萄糖传感微胶囊，并对其响应特性进行了研究。首先，提出了描述传感胶囊信号增强功能的理论模型，并进行了实验验证。其次，设计合成了新型近红外染料作为植入式葡萄糖光学传感器的传感元件。最后，建立了葡萄糖传感微胶囊的制备方法。据此，采用乳液聚合法制备了以氨基为固定化中心的微球。在固定化葡萄糖氧化酶和染料后，采用层层包覆的方法在微球表面覆盖五层聚阳离子/聚阴离子。所制备的葡萄糖传感微胶囊对葡萄糖具有快速、可逆的颜色响应。

In order to develop a minimally invasive sensor system for in vivo monitoring of glucose in interstitial fluid, glucose sensing micro-capsules with a function of signal enhancement based on the regulation of transport of enzymatic products across the capsule membranes had been fabricated and their response properties were investigated. At first, a theoretical model describing signal-enhancement functions of the sensing capsule was proposed and experimentally verified. Second, novel near-infrared dyes were designed and synthesized as a sensory element of implantable optical glucose sensors. Finally, a fabrication method of glucose sensing micro-capsules was established. Accordingly, microspheres having amino moieties as immobilizing sites were prepared using an emulsion polymerization method. After immobilizing enzyme glucose oxidase and the dye, the microspheres were covered with five layers of polycation/polyanion using a layer-by-layer method. The thus prepared glucose sensing micro-capsules showed rapid and reversible color responses for glucose.