Development of novel glucose sensing capsules with a function of signal enhancement

具有信号增强功能的新型葡萄糖传感胶囊的研制

• 批准号: 21550074
• 负责人: TOHDA Koji
• 金额: $ 3.16万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21550074/
• 关键词: バイオセンサー; オプティカル酵素センサー; 微粒子カプセル; 近赤外吸収色素; 分子認識

In order to develop a minimally invasive sensor system for in vivo monitoring of glucose in interstitial fluid, glucose sensing micro-capsules with a function of signal enhancement based on the regulation of transport of enzymatic products across the capsule membranes had been fabricated and their response properties were investigated. At first, a theoretical model describing signal-enhancement functions of the sensing capsule was proposed and experimentally verified. Second, novel near-infrared dyes were designed and synthesized as a sensory element of implantable optical glucose sensors. Finally, a fabrication method of glucose sensing micro-capsules was established. Accordingly, microspheres having amino moieties as immobilizing sites were prepared using an emulsion polymerization method. After immobilizing enzyme glucose oxidase and the dye, the microspheres were covered with five layers of polycation/polyanion using a layer-by-layer method. The thus prepared glucose sensing micro-capsules showed rapid and reversible color responses for glucose.

为了开发一种用于体内监测组织液中葡萄糖的微创传感器系统，制备了具有信号增强功能的葡萄糖传感微胶囊，该微胶囊具有基于调节酶产物跨胶囊膜运输的功能，并研究了其响应特性。首先，提出了描述传感胶囊信号增强功能的理论模型并进行了实验验证。其次，设计并合成了新型近红外染料作为植入式光学葡萄糖传感器的传感元件。最后建立了葡萄糖传感微胶囊的制备方法。因此，使用乳液聚合方法制备具有氨基部分作为固定位点的微球。固定化葡萄糖氧化酶和染料后，采用逐层法在微球上覆盖五层聚阳离子/聚阴离子。由此制备的葡萄糖传感微胶囊对葡萄糖表现出快速且可逆的颜色响应。

项目成果

Enzyme-based optical glucose sensors with rapid and enhanced responses

基于酶的光学葡萄糖传感器具有快速和增强的响应

• 发表时间: 2011
• 作者: 遠田浩司;植村宗佑;安田祭花
• 通讯作者: 安田祭花

シグナル増幅能を内蔵するオプティカルグルコースセンシングカプセルの開発

开发内置信号放大功能的光学葡萄糖传感胶囊

• 发表时间: 2011
• 作者: Koji Tohda;Tatsuya Yamamoto;Miklos Gratzl;Koji Tohda;Koji Tohda;遠田浩司;遠田浩司,植村宗佑
• 通讯作者: 遠田浩司,植村宗佑

Modelling the response function of enzyme-based optical glucose-sensing capsules

模拟基于酶的光学葡萄糖传感胶囊的响应函数

• 发表时间: 2010
• 期刊: Supramolecular Chemistry
• 影响因子: 3.3
• 作者: Koji Tohda;Tatsuya Yamamoto;Miklos Gratzl
• 通讯作者: Miklos Gratzl

Modelling the response function of enzyme-based optical glucose sensing capsules

基于酶的光学葡萄糖传感胶囊的响应函数建模

• 发表时间: 2010
• 期刊: Supramolecular Chemistry
• 影响因子: 3.3
• 作者: Koji Tohda;Tatsuya Yamamoto;Miklos Gratzl;Koji Tohda
• 通讯作者: Koji Tohda

イオン対抽出に基づくオプティカル糖センサーの開発

基于离子对萃取的光学糖传感器的研制

• 发表时间: 2011
• 作者: 遠田浩司;北川文隆;飯野貴充
• 通讯作者: 飯野貴充