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Development of new cross-linkers to detect DNA-protein interaction

Development of new cross-linkers to detect DNA-protein interaction
开发新的交联剂来检测 DNA-蛋白质相互作用
批准号:
21550164
负责人:
WATANABE Soichiro
金额:
$3.16万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011

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项目成果

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英文摘要
Design and synthesis of new cross-linkers having both DNA and protein binding moieties have been developed. Expression vectors for fusion protein with SNAP tag were also established. Binding experiment of new cross-linkers with fusion proteins as well as a DNA duplex was carried out. In both cases, a new band was observed after gel electrophoresis, suggesting that cross-linkers can make a covalent bond with biomolecules of our interest.
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DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [Soichiro Watanabe, Ayako Komori, Masayuki Saito, Akira Uchida]
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DOI: 10.1016/j.bbrc.2011.02.050
发表时间: 2011-03-18
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子: 3.1
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通讯作者: Matsumoto, Kojiro
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [高橋俊樹, 岸本利彦, 渡邊総一郎]
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DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [Y.Shiina, S.Oishi, H.Ishida, 今井友理香・柳内圭子・柳内和幸]
通讯作者: 今井友理香・柳内圭子・柳内和幸
31
    Development of a new efficient methodology to isolate trace amounts of proteins in cells
    • 批准号:
      24550195
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.49万
    • 财政年份:
      2012
    • 负责人:
      WATANABE Soichiro
    • 依托单位: