Development of new cross-linkers to detect DNA-protein interaction
Development of new cross-linkers to detect DNA-protein interaction
批准号:
21550164
负责人:
WATANABE Soichiro
金额:
$3.16万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
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英文摘要
Design and synthesis of new cross-linkers having both DNA and protein binding moieties have been developed. Expression vectors for fusion protein with SNAP tag were also established. Binding experiment of new cross-linkers with fusion proteins as well as a DNA duplex was carried out. In both cases, a new band was observed after gel electrophoresis, suggesting that cross-linkers can make a covalent bond with biomolecules of our interest.
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尿嘧啶取代氮杂大环化合物的制备及反应
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Soichiro Watanabe, Ayako Komori, Masayuki Saito, Akira Uchida]
通讯作者:
Akira Uchida
DOI:
10.1016/j.bbrc.2011.02.050
发表时间:
2011-03-18
期刊:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子:
3.1
作者:
[Ito, Kenichiro, Higai, Koji, Matsumoto, Kojiro]
通讯作者:
Matsumoto, Kojiro
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DOI:
--
发表时间:
2012
期刊:
影响因子:
--
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[高橋俊樹, 岸本利彦, 渡邊総一郎]
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渡邊総一郎
厳密な遺伝子発現誘導ベクターとそれを利用した再生医療関連遺伝子導入ラットの作成
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DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Y.Shiina, S.Oishi, H.Ishida, 今井友理香・柳内圭子・柳内和幸]
通讯作者:
今井友理香・柳内圭子・柳内和幸
Synthesis and Properties of New Crosslinking Reagents to Detect DNA-Protein Interaction
用于检测 DNA-蛋白质相互作用的新型交联试剂的合成和性质
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Soichiro Watanabe, Takuya Toyoshima, Shoi Yamauchi, Keiko Yanai, Takashi Hasegawa, and Kazuyuki Yanai, Design]
通讯作者:
Design
共 31 条
Development of a new efficient methodology to isolate trace amounts of proteins in cells
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批准号:24550195
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.49万
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财政年份:2012
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负责人:WATANABE Soichiro
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依托单位: