Development of novel screening system for enzyme-displaying yeasts using calcium-alginate based beads.

本研究探索了一种新的酵母菌筛选方法。利用最新开发的反胶束方法，将显示酶的酵母细胞分别捕获到微米级海藻酸钙微珠中，以防止水解的荧光底物扩散。对捕获的细胞采用流动分选，成功地实现了活性细胞的富集化。该体系为快速筛选具有多种水解酶活性的酵母细胞提供了一种有效方法。

In this study, a novel screening method for enzyme-displaying yeasts were investigated. Enzyme-displayed yeast cells were captured in the micro-sized calcium alginate beads each by each using newly developed reverse micelle method to prevent diffusion of hydrolyzed fluorescent substrates. Adopting flow sorting to these captured cells, active cells were successfully enriched. This system would be a useful method for high speed screening of yeast cells which display various hydrolyzing enzymes with high activity.