Structural analysis of mammalian sperm flagella with special reference to Tektins

我们已经阐明了Tektin 2，Tektin 3，Tektin 4，Tektin 5在大鼠和小鼠精子中的亚细胞分布。Tektin 2定位于外致密纤维（ODF）周围，Tektin 3定位于线粒体和ODF表面。Tektin 4位于ODF皮质表面，Tektin 5位于鞭毛中段线粒体内表面。通过酵母双杂交系统，我们分离到一个新的与Tekitn 2相互作用的分子Tek 2-BP 1，以及与Tekitn 5相互作用的分子Tektin 4和Odf 1。我们发现Tek 2-BP 1定位于小鼠精子鞭毛的线粒体上。

We have clarified the subcellular distribution of Tektin2, Tektin3, Tektin4, Tektin5 in rat and mouse spermatozoa. Tektin2 is localized around outer dense fiber(ODF), Tektin3 is on the surface of mitochondria and ODF. Tektin4 is on the surface of ODF cortex, and Tektin5 is present on the inner surface of mitochondria in the middle piece od flagella. By yeast Two-Hybrid system, we isolated a novel molecule Tek2-BP1 as Tekitn2-interacting molecule as well as Tektin4 and Odf1 as a Tekitn5-interacting molecule. We found that Tek2-BP1 is localized on mitochondria of mouse sperm flagella.