Sperm recognition and matrix formation mechanisms of mammalian egg coats

哺乳动物卵衣的精子识别和基质形成机制

• 批准号: 21570109
• 负责人: YONEZAWA Naoto
• 金额: $ 3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570109/
• 关键词: 糖タンパク質; 受精; 細胞外マトリクス; 透明帯; 細胞外マトリックス

Protein compositions of mammalian egg coats are different among mammals, and therefore sperm recognition mechanism might be different among mammals. In pigs and cows, both egg coats are composed of ZP2, ZP3, and ZP4 glycoproteins and ZP3/ZP4 complex shows sperm-binding activity. Therefore, we speculated that sperm recognition mechanisms are similar between pigs and cows. In this project, we found that in cows N-terminal region of ZP3 and C-terminal region of ZP4 were involved in sperm-binding activity. On the other hand, in pigs C-terminal region of ZP3 and N-terminal region of ZP4 were involved in the binding activity. This difference in sperm-binding sites suggests difference in supramolecular structures between pig and bovine egg coats. This hypothesis is to be investigated.

哺乳动物卵被蛋白质组成不同，精子识别机制可能也不同。在猪和牛中，两种卵壳均由ZP 2、ZP 3和ZP 4糖蛋白组成，ZP 3/ZP 4复合物显示精子结合活性。因此，我们推测猪和牛的精子识别机制相似。在本项目中，我们发现在奶牛中ZP 3的N端区域和ZP 4的C端区域参与精子结合活性。另一方面，在猪中，ZP 3的C-末端区域和ZP 4的N-末端区域参与结合活性。这种精子结合位点的差异表明猪和牛卵壳之间的超分子结构的差异。这一假设有待研究。

项目成果

Expression, purification and spectroscopic analyses of human reticulocalbin-1

人 reticullocalbin-1 的表达、纯化和光谱分析

• 发表时间: 2009
• 作者: Tomohiro Mizobata;Tatsuya Uemura;Kazuhiro Isaji;Takuma Hirayama;Kunihiro Hongo;Yasushi Kawata;鈴木七緒
• 通讯作者: 鈴木七緒

ブタ透明帯糖タンパク質のジスルフィド結合パターン

猪透明带糖蛋白的二硫键模式

• 发表时间: 2010
• 作者: A.Miyajima;Y.Fukui;K.Wada;尾崎健;尾崎健・中川敦司・長谷俊治・楠木正巳;田中淳平
• 通讯作者: 田中淳平

A forensic method for the simultaneous analysis of biallelic markers identifying Y chromosome haplogroups inferred as having originated in Asia and the Japanese archipelago

• DOI: 10.1016/j.fsigen.2009.06.001
• 发表时间: 2010-02-01
• 期刊: FORENSIC SCIENCE INTERNATIONAL-GENETICS
• 影响因子: 3.1
• 作者: Mizuno, Natsuko;Kitayama, Tetsushi;Kasai, Kentaro
• 通讯作者: Kasai, Kentaro

Sperm binding site on bovine zona pellucida glycoprotein ZP3/ZP4 complex

牛透明带糖蛋白 ZP3/ZP4 复合物上的精子结合位点

• 发表时间: 2009
• 作者: T.Kishino;T.Yonezawa;K.Wada;曲井詩乃
• 通讯作者: 曲井詩乃

Studies on sperm binding sites of bovine zona pellucida glycoproteins ZP3 and ZP4 expressed by baculovirus-expression system

杆状病毒表达系统表达的牛透明带糖蛋白ZP3和ZP4精子结合位点的研究

• 发表时间: 2009
• 作者: Masahiko Takahashi;Kazuki Igarashi;Keisuke Kaneko;Emi Miura;Haimei Wang;Takeshi Ishii;Kenji Kubota;Kaori Wakamatsu;Naoto Yonezawa
• 通讯作者: Naoto Yonezawa