The study of roles of RACK1 in physiological functions of diacylglycerol kinase(type II)

RACK1在二酰甘油激酶(II型)生理功能中的作用研究

• 批准号: 21570145
• 负责人: IMAI Shin-ichi
• 金额: $ 3万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570145/
• 关键词: シグナル伝達; 脂質; 酵素; ジアシルグリセロールキナーゼ; RACK1; 上皮増殖因子; リン酸化; 低分子量GTP結合タンパク質; 腫瘍壊死因子α

Knockdown of diacylglycerol kinase_η(DGK_η) using siRNA inhibited cell proliferation of the HeLa cells. Moreover, DGK_ηactivateed the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by epidermal growth factor(EGF). DGK_ηregulated recruitment of B-Raf and C-Raf from cytosol to membranes and their heterodimerization in EGF-stimulated HeLa cells. DGK_ηalso activated C-Raf but not B-Raf in an EGF-dependent manner. These results suggest that DGK_ηacts as a novel critical regulatory component of the ERK signaling pathway. On the other hand, it is suggested that DGKα positively regulates tumor necrosis factor_α-dependent nuclear factor-_κB activation via the protein kinase Cζ-mediated Ser311 phosphorylation of p65 subunit in AKI melanoma cells.

用小干扰RNA敲除二酰甘油激酶_η(DGK_η)可抑制HeLa细胞的增殖。此外，DGK_η还激活了表皮生长因子诱导的RAS/B-Raf/C-Raf/MEK/ERK信号通路。DGK_η调节表皮生长因子刺激的HeLa细胞中B-Raf和C-Raf从胞浆到膜的募集及其异二聚化。DGK_η也能激活C-Raf，但不能激活B-Raf，且依赖于EGF。这些结果表明，DGKERK_η是ERK信号通路中的一个新的关键调控成分。另一方面，DGKα通过蛋白激酶Cα介导的κ-ζ磷酸化p65亚基，正向调节肿瘤坏死因子依赖的核因子-αAKI B的激活。

项目成果

アルツハイマー病分子マーカーの解析

阿尔茨海默病分子标志物分析

• 发表时间: 2011
• 作者: Arai;S.;相馬仁
• 通讯作者: 相馬仁

Early expression of plasma CCL8 closely correlates with survival rate of acute graft-vs.-host disease in mice.

• DOI: 10.1016/j.exphem.2011.07.006
• 发表时间: 2011-11
• 期刊: Experimental hematology
• 影响因子: 2.6
• 作者: Masaki Yamamoto;Akinobu Ota;T. Hori;S. Imai;H. Sohma;N. Suzuki;N. Hatakeyama;N. Inazawa;Y. Ito;H. Kimura;H. Tsutsumi;Y. Kokai

Investigation of novel biomarkers for Alzheimer's disease using lipid-coated nanoparticles

使用脂质涂层纳米颗粒研究阿尔茨海默病的新型生物标志物

• 发表时间: 2011
• 作者: Sohma;H.
• 通讯作者: H.

Investigation of annexin A5 as a biomarker for Alzheimer's disease using neuronal cell culture and mouse model

• DOI: 10.1002/jnr.22427
• 发表时间: 2010-09
• 期刊: Journal of Neuroscience Research
• 影响因子: 4.2
• 作者: Mami Yamaguchi;Y. Kokai;S. Imai;K. Utsumi;Kyoichi Matsumoto;Hirohito Honda;Y. Mizue;M. Momma

メタロチオネインはCRPS患者の末梢神経で欠損している

CRPS 患者周围神经缺乏金属硫蛋白

• 发表时间: 2011
• 作者: K. M. Mozaffor Hossain;Satoshi Arai;Shinya Saijo;Yoshimi Kakinuma;Takeshi Murata;and Ichiro Yamato;大木豪介
• 通讯作者: 大木豪介