Development of Analytical System of Structure and Function of Membrane Proteins with Surface-enhanced Infrared Spectroscopy

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Experimental techniques on membrane proteins for solubilizing with short-chain phospholipids, reconstituting into partially fluorinated phospholipid vesicles and on a sensitive infrared detection with surface-enhanced effects have been developed for constructing an analytical system of structure and function of membrane proteins. It has been demonstrated that short-chain phospholipids are powerful for solubilization of membrane proteins and that vesicles of partially fluorinated phospholipids are suitable materials for incorporating membrane proteins under native conditions. An analytical system with surface-enhanced infrared spectroscopy has been developed for membrane proteins research.