Molecular mechanism of centromere clustering at SPB in the fission yeast

我们证明了Sad 1的N-末端结构域是S.当细胞从减数分裂周期返回到有丝分裂周期时，动粒组分的有效再积累需要裂殖子，从而促进在SPB处重新建立着丝粒簇。我们还发现，内核膜蛋白Ima 1在着丝粒-SPB相互作用中没有明显的作用，而是需要形成和维持核膜结构和细胞周期进程。

We demonstrated that N-terminal domain of Sad1, an SPB component in S. pombe, is required for efficient re-accumulation of kinetochore components when cells return to mitotic cycle from meiotic cycle and thereby facilitate re-establishment of the centromere clustering at SPB. We also showed that inner nuclear membrane protein Ima1 has no apparent role in the centromere-SPB interaction, but instead is required for the formation and maintenance of nuclear membrane structure and the cell cycle progression.