Study on molecular co-evolution of enzyme and substrate

酶与底物分子协同进化研究

• 批准号: 21570240
• 负责人: YASUMASU Shigeki
• 金额: $ 3.16万
• 依托单位: Sophia University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570240/
• 关键词: 機能進化; 共進化; 孵化酵素; 遺伝子重複; 卵膜; 硬骨魚類; 卵膜タンパク質; 卵膜分解機構; 新規機能遺伝子; 遺伝子; 進化; 酵素

In previous phylogenetic analysis, we suggested that fishes included in Elopomorpha, as basal teleosts, possess a single type of hatching enzyme genes, and that fishes in Otocephala and Euteleostei gain two types of hatching enzyme genes called clade I and II genes by duplication. The Elopomorph hatching enzyme and the clade I enzyme swell the egg envelope by cleaving the N-terminal regions of ZP proteins, while the clade II enzyme solubilizes the swollen envelope by cleaving the two specific sites at N-terminal of ZP domain and the middle region on the ZP domain. In the evolutionary scenario, our findings support that the clade I enzymes inherit the ancestral enzyme function, and the clade II enzymes gain a new function during evolution to Otocephala and Euteleostei. Our study also suggested that a change in the egg envelope protein during evolution strongly contributed to the acquisition of a new function of euteleostean clade II enzyme.

在之前的系统发育分析中，我们认为底硬鱼目的鱼只具有一种类型的孵化酶基因，而尾硬鱼目和真硬鱼目的鱼则通过重复获得两种类型的孵化酶基因，即进化支I和进化支II基因。Elopomorph孵化酶和进化枝I酶通过切割ZP蛋白的n端区域使卵包膜膨胀，而进化枝II酶通过切割ZP结构域n端和ZP结构域中部的两个特定位点使肿胀的卵包膜溶解。在进化过程中，我们的发现支持了进化支系I酶继承了祖先的酶功能，而进化支系II酶在进化到耳头类和真骨类的过程中获得了新的功能。我们的研究还表明，在进化过程中，卵包膜蛋白的变化强烈地促进了中端骨进化枝II酶的新功能的获得。

项目成果

Molecular co-evolution of protease and proteinaceous substrate : hatching enzyme and egg envelope protein as a model system

蛋白酶和蛋白质底物的分子共同进化：孵化酶和卵膜蛋白作为模型系统

• 发表时间: 2011
• 作者: Mari Kawaguchi;Mutsumi Nishida;Shigeki Yasumasu
• 通讯作者: Shigeki Yasumasu

Mechanism of egg envelope digestion by hatching enzymes, HCE and LCE in medaka, Oryzias latipes.

• DOI: 10.1093/jb/mvq086
• 发表时间: 2010-10
• 期刊: Journal of biochemistry
• 影响因子: 2.7
• 作者: S. Yasumasu;M. Kawaguchi;S. Ouchi;K. Sano;K. Murata;H. Sugiyama;T. Akema;I. Iuchi

Crystallization and preliminary X-ray analysis of ZHE1, a hatching enzyme from the zebrafish Danio rerio

• DOI: 10.1107/s1744309109033016
• 发表时间: 2009-10-01
• 期刊: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
• 影响因子: 0.9
• 作者: Okada, Akitoshi;Nagata, Koji;Tanokura, Masaru
• 通讯作者: Tanokura, Masaru

Remarkable consistency of exon-intron structure of hatching enzyme genes and molecular phylogenetic relationships of teleostean fishes

硬骨鱼类孵化酶基因外显子-内含子结构与分子系统发育关系的显着一致性

• DOI: 10.1007/s10641-011-9920-1
• 发表时间: 2012
• 期刊: Environmental Biology of Fishes
• 影响因子: 1.4
• 作者: Mari Kawaguchi;Sebastien Lavoue;Junya Hiroi;Hirofumi Hayano;Ichiro Iuchi;Shigeki Yasumasu;Mutsumi Nishida
• 通讯作者: Mutsumi Nishida

孵化酵素の環境適応～1つのアミノ酸変異による至適塩濃度の違い～

孵化酶的环境适应 ~ 一种氨基酸突变导致的最佳盐浓度差异 ~

• 发表时间: 2011
• 作者: Sano K;Kawaguchi M;Yoshikawa M;Kaneko T;Tanaka T;Iuchi I and Yasumasu S;野村健太郎・川口眞理・竹花佑介・成瀬清・安増茂樹;佐野香織・川口眞理・井内一郎・安増茂樹;川口眞理;川口眞理・井内一郎・西田睦・安増茂樹
• 通讯作者: 川口眞理・井内一郎・西田睦・安増茂樹