Genomic analysis of the S-locus region and identification of the S determinant in wild barley, Hordeum bulbosum

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Hordeum bulbosum, a wild species of barley, possesses self-incompatibility(SI) controlled by unlinked two loci, S and Z. To search for a new candidate gene encoding the pollen S determinant, we conducted sequence analysis of the genomic region around the HPS10 gene, a promising candidate of the pistil S gene. DNA sequences of genomic clone contigs obtained from S1 and S3 haplotypes(50kb and 67kb, respectively) were completely determined and then subjected for prediction of genes and expression analysis. The results showed the presence of polymorphic genomic regions around HPS10 gene between two S haplotypes, where several genes expressed in reproductive tissues were detected. Furthermore, proteome analysis of the pollen proteins combined with the above-mentioned sequence data newly identified putative pollen proteins that may function as a recognition molecule of the SI.