Molecular mechanisms of circadian regulation in CAM

点击翻译按钮获取中文摘要

To elucidate molecular the mechanisms for circadian regulation of CAM, the 5' flanking region of CAM-related genes(ppcD, Ppck, Ppc1, McPpck1, Mod1) were isolated from CAM plants such as Mesembryanthemum crystallinum, Kalanchoe pinnata, K. fedtschenkoi. In the regions of these plants, cis-regulatory elements responsible for ABA, ethylene, drought and salt stresses and circadian regulate and the binding sites of Myb and Dof were found. Transient-expression assays indicated that the sequence 1600 to-1100 of McPpck of M. crystallinum was sufficient to drive the expression of the reporter gene. We applied Agrobacterium-mediated in planta transformation procedure to M. crystallinum. Transformation rates using cotyledons, side shoots and seeds were 20%, 90% and 70%, respectively.