Study of the role of ecdysteroid for insect diapause

蜕皮激素对昆虫滞育作用的研究

• 批准号: 21580070
• 负责人: KIUCHI Makoto
• 金额: $ 3.08万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580070/
• 关键词: 昆虫生理; 休眠; 脱皮ホルモン; 緑きょう病菌; オオワタノメイガ; 脱皮ホルモン分解酵素

Many researches suggested that larval and pupal diapauses are maintained by ecdysteroid deficiency, however, the endocrinological cue to trigger diapause is still not very clear. We therefore examined the role of ecdysteroid for the induction of the larval diapause of Haritalodes basipunctalis. When non-diapausing larvae of H. basipunctalis were injected with an ecdysteroid inactivating enzyme ecdysteroid-22-oxidase(E22O) identified from Nomuraea rileyi, they survived for a few months without eating anything. The E22O-injected larvae also acquired high cold-hardiness, a characteristic often observed in insects in diapause. Furthermore, they resumed development after being exposed to chilling conditions, which is the environmental stimulus to break diapause in many insect species. These developmental and survival characteristics were indistinguishable from those of the normal diapausing larvae, suggesting strongly that the E220 injection induced the larval diapause in H. basipunctalis. To the best of our knowledge, this is the first report indicating that a reduction in the ecdysteroid titer is a sufficient endocrinological stimulus to induce diapauses in insects.

许多研究表明，幼虫和蛹的滞育是通过缺乏表皮甾体激素来维持的，然而，触发滞育的内分泌线索尚不清楚。因此，我们研究了表皮甾体激素在诱导基刺螟幼虫滞育中的作用。给未绝育的基刺棘球绦虫幼虫注射从野孢菌中鉴定的表皮甾体灭活酶-22-氧化酶（E22O），它们在不吃任何东西的情况下存活了几个月。注射了e22o的幼虫也获得了较高的耐寒性，这是昆虫滞育过程中经常观察到的特征。此外，它们在低温条件下恢复发育，这是许多昆虫打破滞育的环境刺激。这些发育和存活特征与正常滞育幼虫的发育和存活特征没有明显区别，表明E220注射诱导了基孔蚜幼虫的滞育。据我们所知，这是第一次有报告表明，蜕皮激素滴度的降低是一个足以诱导昆虫滞育的内分泌刺激。

项目成果

昆虫病原性糸状菌由来の酵素を利用した脱皮ホルモン不活性化技術の開発

利用昆虫病原真菌衍生酶开发蜕皮激素灭活技术

• 发表时间: 2009
• 作者: H.Tamegai;H.Sawada;E.Nagano;Aoki;H.Hirakawa;T.Iino;T.Eguchi;神村学,上野千尋,豊田衣子,志村幸子 & 木内信
• 通讯作者: 神村学,上野千尋,豊田衣子,志村幸子 & 木内信

カビ由来の脱皮ホルモン分解酵素を用いた簡便な脱皮ホルモン濃度低下法の開発

开发一种利用霉菌衍生的蜕皮激素降解酶降低蜕皮激素浓度的简单方法

• 发表时间: 2012
• 作者: 神村学;丹羽隆介;新美輝幸;豊田衣子;上野千尋;志村幸子;木内信
• 通讯作者: 木内信

Purification and characterization of silkworm hemocytes by flow cytometry

• DOI: 10.1016/j.dci.2008.09.005
• 发表时间: 2009-01-01
• 期刊: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
• 影响因子: 2.9
• 作者: Nakahara, Yuichi;Shimura, Sachiko;Kamimura, Manabu
• 通讯作者: Kamimura, Manabu

脱皮ホルモン分解酵素を利用したオオワタノメイガ幼虫の休眠誘導

利用蜕皮激素降解酶诱导棉螟幼虫滞育

• 发表时间: 2010
• 作者: Mase;K.;Okada;E.;Iizuka;T.;Yamamoto;T.;神村学・豊田衣子・上野千尋・木内信
• 通讯作者: 神村学・豊田衣子・上野千尋・木内信

A Eukaryotic (Insect) Tricistronic mRNA Encodes Three Proteins Selected by Context-dependent Scanning

• DOI: 10.1074/jbc.m110.180398
• 发表时间: 2010-11-19
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Kanamori, Yasushi;Hayakawa, Yoichi;Kamimura, Manabu
• 通讯作者: Kamimura, Manabu