Engineering of oxygen-requiring properties in Corynebacterium glutamicum

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We identified six genes relevant to the microaerobic growth of Corynebacterium glutamicum ; Cgl0807, Cgl1102, Cgl0600, Cgl1427, Cgl2857, and Cgl2859. Among these, the Cgl1427 gene encoding cytidylate kinase was shown to play an important role in DNA synthesis when cells were grown under low oxygen concentrations, suggesting that DNA synthesis might become a limiting factor for growth under oxygen limitation. Meanwhile, from the viewpoint of reducing oxygen requirement itself, we engineered a C. glutamicum strain with a modified glycolytic pathway that can generate NADPH instead of NADH, and showed its potential as a host for biotechnological processes.