Studies on imbalance localization of phospholipids in the cellular membrane

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In this study, we clarify the relation between imbalance localization of double-bond in the phospholipids and newly found lyso-phospholipid acyltransferases. Two acyltransferases Lpt1 and Slc1 were purified(or partially purified) and substrate specificities were examined. Also total lipids were extracted from lpt1 or slc1 gene disrupted strain and subjected to LC-MS/MS analysis. From these experiments, Two enzymes mainly catalyze introduction of acyl-residue containing double-bond, although substrate preference were different.