Development of immunopotentiating food material using polysaccharide from discolored laver

我们检测了小鼠巨噬细胞样细胞系（J774. 1)并通过从变色紫菜（可食用紫菜）中纯化的卟啉在小鼠原代脾细胞中的作用。J774中白细胞介素（IL）-12的产生。卟啉、脂多糖（LPS）和热灭活的植物乳杆菌（Lactobacillusplantarum）均能促进细胞增殖。在C3 H/ HeN小鼠（LPS敏感）的原代脾细胞中，卟啉、LPS和L.植物这些结果表明，卟啉诱导T辅助1型细胞促进的细胞介导的免疫应答。因此，从变色紫菜中提取的卟啉可作为功能性食品材料开发，以保护衰老引起的细胞免疫功能下降。此外，卟啉对人小肠Caco-2细胞也有抑制作用。

We examined cytokine productions in mouse macrophage-like cell line(J774. 1) and in mouse primary spleen cells by porphyran purified from discolored nori(edible laver). The production of interleukin(IL)-12 in J774. 1 cells was enhanced by porphyran, lipopolysaccharide(LPS) and heat-killed Lactobacillus plantarum. In primary spleen cells from C3H/ HeN(sensitive to LPS) mice, the productions of IL-12 and interferon(IFN)-γwere enhanced by porphyran, LPS, and L. plantarum. These results suggest that porphyran induces cell-mediated immune response promoted by T helper 1 type cells. Therefore, porphyran from discolored laver can be useful for development as functional food material to protect decrease of cellular immunity in aging. In addition, antiinflammatory effect of porphyran was also showed in human intestinal Caco-2 cells.