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Nucleic acid analysis of causative virus(JEAdV) of the viral endothelial cells necrosis of eel(VECNE) in cultured eel

培养鳗鱼病毒内皮细胞坏死病（VECNE）致病病毒（JEAdV）的核酸分析

基本信息

批准号：
21580235
负责人：
ONO Shin-ichi
金额：
$ 2.5万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

Viral DNA fragments of the virus causing viral endothelial cell necrosis of eel were obtained using rapid determination system for viral nucleic acid sequences(RDV), and 15, 131bp circular full genome sequences were determined using a next-generation sequencing system, overlapping PCR, and Southern blot analysis. One open reading frame(ORF) was homologous to the large T-antigen of polyomavirus ; other ORFs have no homology with any nucleic or amino acid sequences of polyomavirus. Therefore, as this DNA virus might comprise a novel virus family, we provisionally named it Japanese eel endothelial cells-infecting virus(JEECV). The conventional PCR is performed using three different primers. These primer set only amplified in the virus infected eels. The relative percent survival(RPS) of the formalin inactivated vaccine by the inoculation in the peritoneal cavity in the eels showed 66.6% after vaccination for 56 days.

采用病毒核酸序列快速测定系统（RDV）获得了鳗鱼病毒性内皮细胞坏死病毒的DNA片段，采用新一代测序系统、重叠PCR和Southern blot分析确定了15,131bp的环状全基因组序列。其中一个开放阅读框（ORF）与多瘤病毒大t抗原同源；其他orf与多瘤病毒的核酸或氨基酸序列无同源性。因此，由于该DNA病毒可能包含一个新的病毒科，我们暂时将其命名为日本鳗鱼内皮细胞感染病毒（JEECV）。使用三种不同的引物进行常规PCR。这些引物只在病毒感染的鳗鱼中扩增。接种56 d后，经腹腔接种的福尔马林灭活疫苗相对存活率（RPS）为66.6%。