Regulation Mechanisms of the body size in mice

我们分析了DDR2转基因小鼠和ATDC5细胞系的表型，重点是生长和大小控制。在先前的研究中，DDR2 KO小鼠的软骨细胞增殖减少，我们还分析了DDR2在软骨组织中的功能。我们建立了DDR2转基因小鼠，该转基因小鼠只在软骨细胞中特异地过表达，但在转基因小鼠的软骨组织中没有明显的表型。为了了解DDR2在软骨细胞增殖和分化中的作用，我们接下来利用DDR2 miRNA在ATDC5细胞中进行了基因敲除实验。我们发现DDR2可以抑制软骨细胞的增殖和分化，通过DDR2在多种组织中的表达，我们认为DDR2在生殖器官中具有重要的功能。

We analyzed phenotypes of DDR2 transgenic mice and ATDC5 cell lines with a focus on growth and size control. In previous study, cartilage cell proliferation decreased in DDR2 KO mice, and we analyzed also the function of DDR2 in cartilage tissue. We produced Ddr2 cDNA transgenic mice, which were specifically overexpressed only in the cartilage cells, but there were no prominent phenotypes in cartilage tissue of the transgenic mice.To understand the role of Ddr2 in chondrocyte proliferation and differentiation, we next performed knockdown experiments using Ddr2 miRNA in ATDC5 cells. We found that DDR2 might repress cell proliferation and differentiation in cartilage cells.DDR2 is suggested to have important function especially in the reproductive organs by the expression in various tissues.