Functional analysis of implantation specific genes using RNA interference

本研究旨在建立大鼠活体子宫的RNA干扰方法。我们建立了两个植入特异性基因ihh和foxa2作为RNA干扰的靶基因。首先，分析了这两个基因的表达模式和调控机制。在此基础上，将这两个基因的siRNA导入活体大鼠子宫，并通过实时定量RT-PCR验证其调控效果。导入24小时后，基因表达水平与对照组差异无统计学意义。本研究的结果将为建立活体子宫的RNA干扰方法提供基础信息。

The present study was conducted to establish the RNA interference method for living rat uterus. We set up two implantation specific genes, Ihh and Foxa2, as a target gene for RNA interference. Firstly, expressional pattern and regulation mechanism was analyzed for these two genes. Based on the information, siRNA of both genes was introduced into the living rat uterus, and the control effect was verified by real-time RT-PCR. After 24h of the introduction, the expression level of genes was not different from the control groups. The results of the present study will be foundational information for establishing the RNA interference method in the living body uterus.