Functional analysis on EphB and ephrin-B expressing in gastric mucosa and their expression behavior in gastric ulcer and cancer

胃黏膜EphB和ephrin-B表达的功能分析及其在胃溃疡和癌症中的表达行为

• 批准号: 21580367
• 负责人: OGAWA Kazushige
• 金额: $ 3.16万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580367/
• 关键词: EphB; ephrin-B; 胃粘膜上皮; 細胞構築; 境界形成; 噴門部胃粘膜上皮; 無腺部胃粘膜上皮; shRNA; ノックダウン; Eph; ephrin; 胃潰瘍; 胃癌

Several EphB receptors and ephrin-Bs were detected in the gastric mucosa of the adult rodent stomach by RT-PCR amplification. Immunostaining showed complementary expression patterns, with EphB receptors preferentially expressed in the deeper regions and ephrin-Bs in the superficial regions of the gastric units. In a mouse ulcer model EphB2 expression was upregulated in the regenerating epithelium. EphB signaling in primary gastric epithelial cells promoted cell retraction and repulsion at least in part through RhoA activation. Based on these findings the author propose that the EphB-positive progeny of gastric stem cells migrates from the isthmus towards the bottom of the gastric glands due to repulsive signals arising from contact with ephrin-Bs, which are preferentially expressed in the more superficial regions of the isthmus and gastric pits. In a rat gastric cancer model EphB2 and ephrin-B were co-localized in Ki67-positive proliferative cells forming aberrant gastric units and gastric cancer cells were simultaneously expressed EphB receptors and ephrin-B ligands. These findings suggest that simultaneous expressions of both EphB receptors and ephrin-B indicates an characteristic of gastric cancer cells.

通过RT-PCR扩增，在成年啮齿动物胃粘膜中检测到多种EphB受体和Ephin-B。免疫组织化学染色呈互补表达模式，EphB受体优先表达于胃单位深层，Ephin-B受体优先表达于胃单位浅表区域。在小鼠溃疡模型中，EphB2在再生上皮中表达上调。原代胃上皮细胞中的EphB信号促进了细胞的回缩和排斥，至少部分是通过RhoA的激活。根据这些发现，作者认为EphB阳性的胃干细胞子代由于与Ephin-B接触而产生的排斥信号而从峡部向胃腺底部迁移，这些信号优先表达于峡部和胃小窝的较浅区域。在大鼠胃癌模型中，EphB2和Ephin-B共定位于Ki67阳性的增殖细胞中，形成异常的胃单位，胃癌细胞同时表达EphB受体和Ephin-B配体。这些发现表明，EphB受体和Ephin-B的同时表达表明了胃癌细胞的特征。

项目成果

再生および異常増殖胃粘膜上皮のEphBとephrin-B発現

再生和异常增殖胃粘膜上皮中 EphB 和 ephrin-B 的表达

• 发表时间: 2009
• 作者: 内山慎太郎;石井万幾;中島崇行;小川和重
• 通讯作者: 小川和重

Complementary expression of EphB receptors and ephrin-B ligands in the pyloric and duodenal epithelium of adult mice

成年小鼠幽门和十二指肠上皮中 EphB 受体和肝配蛋白 B 配体的互补表达

• 发表时间: 2011
• 期刊: Histochem Cell Biol
• 影响因子: 2.3
• 作者: Ishii M;Nakajima T;Ogawa K.
• 通讯作者: Ogawa K.

心筋のEphBとギャップ結合

心肌 EphB 和间隙连接

• 发表时间: 2010
• 作者: ミュラー樹;石井万幾;中島崇行;小川和重
• 通讯作者: 小川和重

組織の細胞構築を制御する分子Ephとephrin : EphB/ephrin-Bは胃粘膜上皮のポジショニングを制御する分子システムであるか?

控制组织细胞结构的 Eph 和 ephrin 分子：EphB/ephrin-B 是控制胃粘膜上皮定位的分子系统吗？

• 发表时间: 2009
• 作者: 武本奈津枝;中島崇行;小川和重;小川和重
• 通讯作者: 小川和重

EphB2 and ephrin-B1 expression in the boundary between the glandular and nonglandular epithelium of the rodent stomach

EphB2和ephrin-B1在啮齿动物胃腺体和非腺体上皮之间边界的表达

• 发表时间: 2012
• 作者: Kazushige Ogawa;Yasutaka Igura;Yuta Hayashi;Noritaka Saeki;Maki Ishii and Takayuki Nakajima
• 通讯作者: Maki Ishii and Takayuki Nakajima