Development of clinical therapeutic methods against failure of fertilizing functions causing summer infertility in boars

公猪夏季不孕症受精功能障碍临床治疗方法的建立

• 批准号: 21580389
• 负责人: MURASE Tetsuma
• 金额: $ 3.24万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580389/
• 关键词: 臨床繁殖; 産科精子; ブタ; 夏季不妊; 精子; 受精; 先体反応; ハイパーアクチベーション; 希釈液; シクロオキシゲナーゼ; 夏季不妊症; 臨床; イオノホア

In an attempt to improving boar sperm quality which decreases in summer and stable provision with semen leading to stable production of pork during the year, this study investigated a new diluent for boar semen storage, a new method to evaluate frozen-thawed bull sperm quality and development of a method to improve fertilizing ability of frozen-thawed bull spermatozoa. Bull spermatozoa were used as a reference to boar spermatozoa. Modified diluents for boar semen improved conception rates. In bulls, new method for sperm evaluation was found out and modification of diluent for freezing bull semen enabled improvement of sperm fertilizing ability.

为了改善夏季下降的公猪精子质量和稳定的精液供应，使全年猪肉产量稳定，本研究探索了一种新的猪精液保存稀释剂，一种新的评价冷冻解冻公牛精子质量的方法，并开发了一种提高冷冻解冻公牛精子受精能力的方法。以公牛精子为参照物，以猪精子为参照。改良的猪精液稀释剂提高了受孕率。在公牛中找到了新的精子鉴定方法，改良了公牛精液冷冻稀释液，提高了精子受精能力。

项目成果

Relationship of Protein Tyrosine Phosphorylation State With Tolerance to Frozen Storage and the Potential to Undergo Cyclic AMP-Dependent Hyperactivation in the Spermatozoa of Japanese Black Bulls

• DOI: 10.1002/mrd.21233
• 发表时间: 2010-10-01
• 期刊: MOLECULAR REPRODUCTION AND DEVELOPMENT
• 影响因子: 2.5
• 作者: Harayama, Hiroshi;Nishijima, Kazuhiro;Fukushima, Moriyuki
• 通讯作者: Fukushima, Moriyuki

Dilution ofboar ejaculates with BTScontaining HEPES in place ofbicarbonate immediately afterejaculation can reduce the increasedinducibility of the acrosome reactionby treatment with calcium and calciumionophore A23187, which ispotentially associated with

射精后立即用含有 HEPES 的 BTS 代替碳酸氢盐稀释公猪精液，可以通过钙和钙离子载体 A23187 处理来减少顶体反应的诱导性增加，这可能与

• 发表时间: 2010
• 期刊: J. Reprod. Dev
• 作者: Murase T;Imaeda N;Yamada H;TakasuM;Taguchi K;Katoh T
• 通讯作者: Katoh T

Dilution of boar ejaculates with BTS containing HEPES in place of bicarbonate immediately after ejaculation can reduce the increased inducibility of the acrosome reaction bytreatment with calcium and calcium ionophore A23187, which is potentially associat

射精后立即用含有 HEPES 的 BTS 代替碳酸氢盐稀释公猪精液，可以通过钙和钙离子载体 A23187 处理来减少顶体反应的诱导性增加，这可能与

• 发表时间: 2010
• 期刊: Journal of Reproduction and Development
• 影响因子: 1.8
• 作者: Doungjit Kanungpean;Tsutomu Kakuda;Shinji Takai;Tetsuma MURASE
• 通讯作者: Tetsuma MURASE

Dilution of boar ejaculates with BTS containing HEPES in place of bicarbonate immediately after ejaculation can reduce the increased inducibility of the acrosome reaction by treatment with calcium and calcium ionophore A23187, which is potentially associa

射精后立即用含有 HEPES 的 BTS 代替碳酸氢盐稀释公猪精液，可以通过钙和钙离子载体 A23187 处理来减少顶体反应的诱导性增加，这可能是相关的

• 发表时间: 2010
• 期刊: Journal of Reproduction and Development (In press)
• 作者: Doungjit Kanungpean;Tsutomu Kakuda;Shinji Takai;Tetsuma MURASE
• 通讯作者: Tetsuma MURASE

Desalting of boar seminal plasma by gel filtration

凝胶过滤法对公猪精浆进行脱盐

• 发表时间: 2010
• 作者: Murase;T.;Imaeda;N.;Yamada;H.;Harayama;H.;Takasu;M.;Taguchi;K.;and Katoh;T.
• 通讯作者: T.