Functional analysis of free N-glycans involved in regulation of plant differentiation.

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In developing plant tissues, free N-glycan that are enzymatically liberated from glycoproteins occur ubiquitously at micromolar level. As a part of study to elucidate the physiological function(s) of these free N-glycans involved in plant development, we constructed knock-out or knock-down plants, in which the expressions of ENGase or PNGase genes were suppressed. The structural features of free N-glycans expressed in those transgenic plants were clearly distinguishable from those expressed in wild type plants. Although significant difference in phenotype between the transgenic plants and wild type was not observed, a specific difference in the fruit-ripening process was observed.