Development of Molecular Species-Specific Live-Single Cell MS with Affinity-modified Nanospray Tips.

我们建立了活单细胞质谱法，它能够在直接质谱分析后，通过金属涂层玻璃尖端澄清单细胞的详尽分子含量。在这项研究中，我们旨在通过修饰玻璃尖端的内表面来提高灵敏度和分子种类。我们用亲水性或疏水性处理来制作针尖，分别允许选择性捕获极性化合物或亲脂性化合物。我们证明了处理过的尖端可以捕获标准化合物和单细胞中的分子。因此，使用该尖端，可以通过活单细胞质谱法检测具有特定物理性质的分子。

We established Live Single-cell Mass Spectrometry which enables to clarify exhaustive molecular content of single cell recovered by a metal-coated glass tip following direct mass spectrometric analysis. In this study, we aimed to increase sensitivity and kinds of molecules by modifying the inner surface of the glass tips. We made the tips with hydrophilic or hydrophobic treatment which allow selective trapping of polar compounds or lipophilic compounds respectively. We proved treated tips to trap standard compound as well as molecules contained in single cell. Thus, detection of molecules with specific physical property can be detected by Live Single-cell Mass Spectrometry using this tip.