Development of an accurate analysis method of the intestinal bacterial flora, and detect the causative bacteria of ulcerative colitis, search its effective antibiotics.

采用DGGE方法对UC模型小鼠和健康小鼠粪便中的总DNA进行分析。结果发现了一些新的UC小鼠特异性条带。此外，我们可以从每个标本中获得15，000 ~ 40，000个序列读数（Roche 454-FLX-Titanium），并在UC模型小鼠中发现某些细菌数量增加。口服从UC模型小鼠中鉴定的代表性微生物Clostridium sp. ID 4菌株后，我们可以在大肠下部发现炎症图像。从这些数据中，结论：本研究发现的某些细菌与UC的发病有一定的关系。

Total DNA extracted from the fecal sample of UC model mouse and the healthy mouse were analyzed by DGGE method. As a result, we could find some new bands which were specific for UC model mouse. Furthermore we could obtain 15,000～40,000 sequence read from each specimen by pyrosequencer(Roche 454-FLX-Titanium), and find the certain bacteria whose population was increasing in UC model mouse.After orally given Clostridium sp. ID4 strain-one representative organism which was identified from UC model mouse-, we could find the inflammatory image in the lower portion of the large intestine.From these data, we concluded that the certain bacteria founding this study were not a little related with the UC onset.