Application of photoactivated adenylyl cyclase to study the mechanisims of neurite formation by controlling temporal and spacial intracellular cAMP level in vitro

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Potencies of the photo-activated adenylyl cyclases(PAC) from euglena and bacteria for controlling intracellular cAMP concentration in vitro were analyzed biochemically with an cAMP-ELISA as well as cell-biologically with an Epac FRET sensor probe. It was also observed that melanosomes labeled with its associated protein Rab27a fused to fluorescent proteins were immediately moved toward peripheral regions of the B16 melanoma cells in response to an increase of the intracellular cAMP. We have also tried to utilize the PACs to study the morphological development and functions of neural cells in PC12 cells and hippocampal primary culture neurons.