A clonality analysis of breast cancer tissue with heterogenity : clarifying the cell lineage for adequate treatment strategy for recurrent tumor

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Aim : To clarify the cell lineage of breast cancer cells the present study was performed. It is significant to understand which cancer cells were recurrent from the initial breast cancer tissue with heterogeneity. The final goal of this study is to provide information for the adequate treatment of recurrent breast cancer.Materials and methods : Formalin-fixed paraffin-embedded sections of the lymph node, the surrounding mammary gland and breast cancer tissue from the patients who had second breast tumors in ipsilateral and contralateral mammary gland after the treatment for initial breast cancer. Two analysis methods, polymorphism of human androgen receptor(HUMARA) and gene alteration of mitochondrial D-loop somatic mutation(GAMDDL) were compared.Results and conclusion : The informative rate of analyses was 42.1%, 76.9% and 89.5% by HUMARA, GAMDDL, and combined HUMARA and GAMDDL, respectively. GAMDDL is a useful method to clarify the cell lineage of cancer cells.